2018—2019年赤道几内亚Bioko岛恶性疟原虫抗药性基因多态性分析  

Drug-resistant gene polymorphisms in Plasmodium falciparum isolated from Bioko Island,Equatorial Guinea in 2018 and 2019

作　　者：何金泉[1,2] 陈江涛 李敬河 陈伟忠[5] 梁雪雁 黄慧莹 韦华贵 黄维益 王俊利 林敏 杨培奎[5] 陈新瑶[5] 刘祥治 HE Jin-Quan;CHEN Jiang-Tao;LI Jing-He;CHEN Wei-Zhong;LIANG Xue-Yan;HUANG Hui-Ying;WEI Hua-Gui;HUANG Wei-Yi;WANG Jun-Li;LIN Min;YANG Pei-Kui;CHEN Xin-Yao;LIU Xiang-Zhi(Department of Laboratory Medicine,Humen Hospital of Dongguan City,Guangdong Province,Dongguan 523000,China;The Chinese Medical Aid Team to the Republic of Equatorial Guinea,Guangdong Province,China;Department of Laboratory Medicine,Huizhou Central Hospital,Guangdong Province,China;Department of Laboratory Medicine,Shijie Hospital,Dongguan City,Guangdong Province,China;Chaozhou People’s Hospital Affiliated to Shantou University,China;School of Laboratory Medicine,Youjiang Medical University for Nationalities,China)

机构地区：[1]广东省东莞市虎门医院检验科,东莞523000 [2]广东援助赤道几内亚医疗队 [3]广东省惠州市中心人民医院检验科 [4]广东省东莞市石碣医院检验科 [5]汕头大学附属潮州市人民医院 [6]右江民族医学院检验学院

出　　处：《中国血吸虫病防治杂志》2021年第4期396-400,共5页Chinese Journal of Schistosomiasis Control

基　　金：广西壮族自治区自然科学基金重点项目(2019JJD140052);广西壮族自治区自然科学基金面上项目(2020JJA140656);广东省教育厅重点学科项目(2019GDXK0031)。

摘　　要：目的调查赤道几内亚Bioko岛恶性疟原虫多药耐药蛋白1(Plasmodium falciparum multidrug resistance protein1,PfMDR1)、氯喹抗性转运蛋白基因(P.falciparum chloroquine resistant transporter,PfCRT)和Kelch 13基因(P.falciparum Kelch 13,PfK13)多态性,为当地疟疾防控策略制定提供参考。方法采集2018—2019年赤道几内亚Bioko岛恶性疟原虫感染者外周血样本85份,提取基因组DNA。采用巢式PCR技术扩增、PfCRT和基因,对扩增产物进行DNA测序,并对基因序列进行比对。结果赤道几内亚Bioko岛恶性疟原虫基因不存在已知与青蒿素抗性相关的突变;PfMDR1基因和PfCRT基因均存在不同比例抗药性突变,其中PfMDR1N86Y、PfMDR1Y184F和PfCRTK76T突变率分别为35.29%(30/85)、72.94%(62/85)和24.71%(21/85)。结论赤道几内亚Bioko岛恶性疟原虫PfMDR1、PfCRT基因和基因均存在不同程度突变。Objective To investigate the genetic polymorphisms of Plasmodium falciparum multidrug resistance protein 1(PfMDR1),chloroquine resistance transporter(PfCRT)and Kelch 13(PfK13)genes in Bioko Island,Equatorial Guinea,so as to provide insights into the development of the malaria control strategy in local areas.Methods A total of 85 peripheral blood samples were collected from patients with Plasmodium falciparum infections in Bioko Island,Equatorial Guinea in 2018 and 2019,and genomic DNA was extracted.The PfMDRl,PfCRT and PfK13 genes were amplified using a nested PCR assay.The amplification products were sequenced,and the gene sequences were aligned.Results There were no mutations associated with artemisinin resistance in PfK13 gene in Bioko Island,Equatorial Guinea,while drug-resistant mutations were detected in PfMDRl and PfCRT genes,and the proportions of PfMDRlN86 Y,PfMDRlY184 F and PfCRTK76 T mutations were 35.29%(30/85),72.94%(62/85)and 24.71%(21/85),respectively.Conclusion There are mutations in PfMDR1,PfCRT and PfK13 genes in P.falciparum isolates from Bioko Island,Equatorial Guinea.

关 键 词：恶性疟原虫 抗药性 基因多态性 赤道几内亚 

分 类 号：R382.3[医药卫生—医学寄生虫学]