Enzymatic Synthesis of a Diastereomer of Neoabyssomicin Derivative Using the Diels-Alderase AbyU  

作　　者：Wenjuan Ding Changbiao Chi Xiaoyi Wei Changli Sun Jiajia Tu Ming Ma Qinglian Li Jianhua Ju 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,RNAM Center for Marine Microbiology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,164 West Xingang Road,Guangzhou,Guangdong,510301 China [2]College of Oceanology,University of Chinese Academy of Sciences,19 Yuquan Road,Beijing,100049 China [3]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),No.1119,Haibin Rd.,Nansha District,Guangzhou,Guangdong,510301 China [4]State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing,100191 China [5]Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong,510650 China

出　　处：《Chinese Journal of Chemistry》2021年第7期1871-1877,共7页中国化学（英文版）

基　　金：This work was supported by the China NSF(Nos.31670087 and U1706206);the Natural Key Research and Development Program of China(No.2019YFC0312500);the Key Special Project for Introduced Talents Team of Southern Marine Science and Engi-neering Guangdong Laboratory(Guangzhou,No.GML2019ZD0406);the Guangdong NSF(No.2016A030312014);the Guangdong Local Innovation Team Program(No.2019BT02Y262);the Pearl River S&T Nova Program of Guangzhou No.201806010109);the CAS(No.XDA13020302-2);the Guangdong Provincial-Level Special Funds for Promoting High-quality Economic Development(No.2020032).

摘　　要：Main observation and conclusion The enzyme AbyU catalyses a Diels-Alder(DA)reaction during abyssomicin C biosynthesis.In this study,AbyU is shown to convert the native substrate of another Diels-Alderase(DAase),AbmU,to a new abyssomicin derivative,abyssomicin 7.

关 键 词：BIOSYNTHESIS Bioorganic chemistry Enzyme catalysis Neoabyssomicin Diels-Alderase 

分 类 号：O62[理学—有机化学]