A boronate-modified renewable nanointerface for ultrasensitive electrochemical assay of cellulase activity  

作　　者：Tianxiang Wei Qiao Xu Caiyu Zou Zeqiang He Yidan Tang Tao Gao Min Han Zhihui Dai 

机构地区：[1]Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,Jiangsu Key Laboratory of Biofunctional Materials,School of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210023,China [2]School of Environment,Nanjing Normal University,Nanjing 210023,China [3]Jiangsu Key Laboratory for Molecular and Medical Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210023,China

出　　处：《Chinese Chemical Letters》2021年第4期1470-1474,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.21625502,21705079,21671105 and 21974070);the Natural Science Foundation of Jiangsu Province (Nos.BK20192008 and BK20171033);the financial support from the PAPD。

摘　　要：The saccharification of cellulosic biomass to produce biofuels and chemicals is one of the most promising industries for gree n-power production and sustainable development.Cellulase is the core component in the saccharification process.Simple and efficient assay method to determine cellulase activity in saccharification is thus highly required.In this work,a boronate-affinity surface based renewable and ultrasensitive electrochemical sensor for cellulase activity determination has been fabricated.Through bo ronate-sugar interaction,celluloses are attached to the electrode surface,forming the cellulose na nonetwork at the sensing interface.Cellulase degradation can lead to the variation of electrochemical impedance.Thus,electrochemical impedance signal can reflect the cellulase activity.Importantly,via fully utilizing the boronate-affinity chemistry that enables reversible fabrication of cellulose nanonetwork,a renewable sensing surface has been firstly constructed for cellulase activity assay.Thanks to interfacial diffusion process of electrochemical sensor,the product inhibitory effect in the cellulase activity assays can be circumvented.The proposed electrochemical sensor is ultrasensitive for label-free cellulase activity detection with a very simple fabrication process,showing great potential for activity screen of new enzymes in saccharification conversion.

关 键 词：Electrochemical biosensor Renewable interface Boronate-sugar interaction Cellulose hydrolysis Cellulase activity 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O657.1[自动化与计算机技术—控制科学与工程]