MMP1及TIMP1基因多态性与儿童反复呼吸道感染的关联性  

Association of polymorphisms of MMP1 and TIMP1 genes with recurrent respiratory tract infection in children

作　　者：侯申岩 邓丹 杨艳新 HOU Shen-yan;DENG Dan;YANG Yan-xin(Chaoyang Central Hospital,Chaoyang,Liaoning 122000,China)

机构地区：[1]朝阳市中心医院检验科,辽宁朝阳122000

出　　处：《中华医院感染学杂志》2021年第16期2457-2461,共5页Chinese Journal of Nosocomiology

基　　金：辽宁省自然科学基金资助项目(20198157)。

摘　　要：目的探究反复呼吸道感染(RRTI)风险和疾病发展与基质金属蛋白酶1(MMP1)、组织金属蛋白酶抑制物1(TIMP1)基因多态性的关系。方法选取2016年5月-2019年12月朝阳市中心医院确诊为RRTI患儿82例和同期体检健康儿童60名作为研究对象,采用聚合酶链式反应和限制性片段长度多态性技术,检测MMP1(-1607位点)、TIMP1(-372位点)基因多态性,分析不同基因型与RRTI的关系;采用酶联免疫吸附法检测外周血MMP1、TIMP1,分析血清MMP1、TIMP1对RRTI的预测价值。结果存在被动吸烟、挑食和服用抗生素>7次/年是RRTI的独立危险因素(P<0.05);RRTI组MMP1、TIMP1高于对照组(P<0.05);受试者工作特征(ROC)分析显示,血清MMP1、TIMP1的曲线下面积(AUC)分别为0.766(95%CI为0.686~0.846)、0.784(95%CI为0.704~0.863);两组MMP1(-1607位点)基因型分布的比较,差异具有统计学意义(P<0.05),与对照组比较,RRTI组MMP1(-1607位点)2G等位基因频率明显升高(P<0.05);两组TIMP1(-372位点)基因型分布的比较,差异无统计学意义,但与对照组比较,RRTI组TIMP1(-372位点)C等位基因频率明显升高(P<0.05)。结论MMP1(-1607位点)2G等位基因、TIMP1(-372位点)C等位基因是RRTI的危险因素,且被动吸烟、挑食和服用抗生素均会增加RRTI风险,临床应给予针对性管理措施,积极防控RRTI。OBJECTIVE To explore the relationship between the risk of recurrent respiratory tract infection(RRTI)and the disease development and polymorphisms of matrix metalloproteinase-1(MMP1)and tissue inhibitor of metalloproteinase-1(TIMP1)genes.METHODS During the period from May 2016 to Dec 2019,82 children with RRTI and 60 healthy children who underwent physical examination in Chaoyang Central Hospital were enrolled.The polymorphisms of MMP1(-1607 locus)and TIMP1(-372 locus)genes were detected by polymerase chain reaction-restriction fragment length polymorphism,and the relationship between different genotypes and RRTI was analyzed.The peripheral blood levels of MMP1 and TIMP1 were detected by enzyme-linked immunosorbent assay.The predictive value of serum MMP1 and TIMP1 for RRTI was analyzed.RESULTS Passive smoking,picky eater and taking antibiotics more than 7 times/year were the independent risk factors for RRTI(P<0.05).MMP1 and TIMP1 levels in the RRTI group were significantly higher than those in the control group(P<0.05).The receiver operating characteristic(ROC)curves analysis showed that area under the ROC curve(AUC)values of serum MMP1 and TIMP1 levesls were 0.766(95%confidence interval:0.686-0.846)and 0.784(95%confidence interval:0.704-0.863),respectively.There were significant differences in the distribution of MMP1 genotype(-1607 locus)between the two groups(P<0.05).Compared with control group,the frequency of 2 G allele at MMP1(-1607 locus)in the RRTI group was significantly increased(P<0.05).There was no significant difference in the distribution of TIMP1(-372 locus)genotype between the two groups;while compared with control group,the frequency of C allele at TIMP1(-372 locus)in the RRTI group was significant higher(P<0.05).CONCLUSION 2 G allele at MMP1(-1607 locus)and C allele at TIMP1(-372 locus)are risk factors of RRTI.The passive smoking,picky eating and taking antibiotics will increase the risk of RRTI.Clinically,targeted management measures should be performed to prevent RRTI effectively.

关 键 词：基质金属蛋白酶1 组织金属蛋白酶抑制物1 反复呼吸道感染 基因多态性 

分 类 号：R725.6[医药卫生—儿科]