儿童肥胖症合并肺炎支原体感染TLR4/NF-κB信号通路与致炎细胞因子水平的关系  

Relationship between TLR4/NF-κB signaling pathway and levels of inflammatory cytokines in obesity children combined with Mycoplasma pneumoniae infection

作　　者：刘超 殷紫 许国莹 李靖 徐秋节 胡得飞[3] LIU Chao;YIN Zi;XU Guo-ying;LI Jing;XU Qiu-jie;HU De-fei(Jiangsu College of Nursing,Huaian,Jiangsu 223005,China;不详)

机构地区：[1]江苏护理职业学院医学技术学院,江苏淮安223005 [2]江苏护理职业学院药学与中药学院,江苏淮安223005 [3]淮安市第二人民医院检验科,江苏淮安223002

出　　处：《中华医院感染学杂志》2021年第16期2522-2526,共5页Chinese Journal of Nosocomiology

基　　金：江苏省高等学校自然科学研究基金面上项目(19KJD360001);淮安市科技基金资助项目(HAP202004)。

摘　　要：目的探讨肥胖症合并肺炎支原体(MP)感染患儿Toll样受体4/核因子-κB(TLR4/NF-κB)信号通路与致炎细胞因子水平的相关性。方法选取2018年5月-2020年8月淮安市第二人民医院收治的68例肥胖症合并MP感染患儿作为研究组;另收集同期收治的肥胖症患儿60例为肥胖组,以及健康体检的健康儿童60名为健康组,检测和比较三组外周血TLR4、NF-κB、NF-κB抑制蛋白(IκB)mRNA相对表达量和血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)水平,分析TLR4、NF-κB、IκB与血清致炎细胞因子IL-6、IL-8、TNF-α之间的关系;并采用单因素、多因素Logisitic回归分析肥胖症患儿发生MP感染的危险因素。结果三组TLR4、NF-κB、IκB mRNA相对表达量和血清IL-6、IL-8、TNF-α水平均有统计学差异(P<0.05),研究组和肥胖组TLR4、NF-κB mRNA相对表达量及血清IL-6、IL-8、TNF-α水平均高于健康组,IκB mRNA低于健康组(P<0.05),且研究组TLR4、NF-κB mRNA相对表达量高于肥胖组,IκB mRNA低于肥胖组;相关性分析结果显示,肥胖症合并MP感染患儿TLR4、NF-κB表达分别与IL-6、IL-8和TNF-α呈正相关性(P<0.05),IκB与IL-6、IL-8、TNF-α均呈负相关性(P<0.05)。多因素分析结果显示,年龄3~7岁、处于低补体状态和有流行接触史是肥胖症患儿发生MP感染的独立危险因素(P<0.05)。结论肥胖症合并MP感染患儿TLR4/NF-κB信号通路处于过度活化状态,并可能通过该途径促进IL-6、IL-8、TNF-α等致炎细胞因子释放,诱导发生肺部炎症反应。OBJECTIVE To explore the correlation between Toll-like receptor 4/nuclear factor-κB(TLR4/NF-κB)signaling pathway and levels of inflammatory cytokines in children with obesity and Mycoplasma pneumonia(MP)infection.METHODS A total of 68 children with obesity and MP infection admitted to Huaian Second People’s Hospital were enrolled as the study group between May 2018 and Aug 2020;60 obesity children admitted to the hospital and 60 healthy children undergoing physical examination during the same period were enrolled as the obesity group and healthy group,respectively.The relative mRNA expression levels of TLR4,NF-κB and NF-κB inhibitory protein(IκB)in peripheral blood,and levels of serum interleukin-6(IL-6),interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)were detected and compared among the three groups.The relationship between levels of TLR4,NF-κB and IκB and serum inflammatory cytokines such as IL-6,IL-8 and TNF-αwas analyzed.The risk factors for MP infection in obesity children were analyzed by univariate and multivariate Logistic regression analysis.RESULTS There were significant differences in the relative mRNA expression levels of TLR4,NF-κB and IκB,and levels of serum IL-6,IL-8 and TNF-αamong the three groups(P<0.05).The relative mRNA expression levels of TLR4 and NF-κB,and levels of serum IL-6,IL-8 and TNF-αin the study group and obesity group were significantly higher than those in the healthy group,while IκB mRNA was significantly lower than that in the health group(P<0.05).The relative mRNA expression levels of TLR4 and NF-κB in the study group were significantly higher than those in the obesity group,while IκB mRNA was significantly lower than that in the obesity group(P<0.05).The correlation analysis showed that the expressions of TLR4 and NF-κB were significantly positively correlated with IL-6,IL-8 and TNF-α(P<0.05),while IκB was significantly negatively correlated with them(P<0.05).Multivariate Logistic regression analysis showed that age within 3-7 years old,low complemen

关 键 词：儿童 肥胖症 肺炎支原体感染 Toll样受体4/核因子-κB信号通路 致炎细胞因子 表达水平 

分 类 号：R723.14[医药卫生—儿科]