嗜酸性粒细胞浸润性鼻黏膜炎症SUMO特异性蛋白酶3表达降低促进替代激活巨噬细胞极化  

Decrease of the SUMO specific protease 3 promotes the alternative activated macrophage polarization in the nasal mucosal inflammation infiltrated with eosinophil

作　　者：暴喜明 周争[1] 李吉平[1] BAO Ximing;ZHOU Zheng;LI Jiping(Department of Otorhinolaryngology,Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属仁济医院耳鼻咽喉科,上海200127

出　　处：《中国眼耳鼻喉科杂志》2021年第5期360-366,共7页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：上海自然科学基金委员会(18ZR1423300);浦东新区卫生和计划生育委员会(PW2017D-2)。

摘　　要：目的探讨SUMO特异性蛋白酶3(SENP 3)在嗜酸性粒细胞浸润性鼻黏膜炎症中对巨噬细胞极化产生的影响。方法嗜酸性粒细胞浸润性鼻黏膜炎症患者鼻黏膜组织中CD68、CD206、SENP3免疫荧光染色共定位。用白细胞介素-4(IL-4)和IL-13刺激SENP3基因敲除小鼠和对照小鼠骨髓来源巨噬细胞(BMDM),体外诱导巨噬细胞极化。利用SENP3基因敲除小鼠建立嗜酸性粒细胞浸润性鼻黏膜炎症动物模型,用免疫荧光共定位检测F4/80、CD206。结果在SENP3基因敲除小鼠建立的嗜酸性粒细胞浸润性鼻黏膜炎症模型中,鼻黏膜中CD206^(+)细胞数量显著增加。在体外诱导BMDM极化过程中,敲除SENP3使F4/80^(+)CD206^(+)细胞数量增加。同时,在对照组小鼠提取的BMDM极化的过程中,SENP3蛋白表达量明显下降。在嗜酸性粒细胞浸润性鼻黏膜炎症患者鼻黏膜内检测到不表达SENP3的CD68^(+)CD206^(+)细胞数量显著多于表达SENP3的CD68^(+)CD206^(+)细胞。结论在嗜酸性粒细胞浸润性鼻黏膜炎症中,巨噬细胞SENP3表达的下调促进替代激活巨噬细胞的极化。Objective To investigate the effect of SUMO-specific protease 3(SENP 3)on macrophage polarization in the nasal mucosal inflammation infiltrated with eosinophil.Methods CD68-CD206-SENP3 immunofluorescence staining was used for co-localization of IL-4 and IL-13 in the nasal mucosa of patients with nasal mucosal inflammation infiltrated with eosinophil.Il-4 and IL-13 were used to stimulate bone marrow derived macrophage(BMDM)of SENP3 gene knockout mice and control mice,and to induce macrophage polarization in vitro.Animal model of the nasal mucosal inflammation infiltrated with eosinophil was established by SENP3 gene knockout mice,and F4/80 CD206 was detected by immunofluorescence co-localization.Results In the nasal mucosal inflammation infiltrated with eosinophil model established in SENP3 knockout mice,the number of CD206^(+)cells in nasal mucosa was increased.For in vitro induced macrophage polarization,SENP3 knockout increased the number of F4/80^(+)CD206^(+)cells.At the same time,SENP3 expression was decreased during the polarization of BMDM extracted from control mice.In patients with eosinophilic chronic sinusitis,the number of CD68^(+)CD206^(+)cells not expressing SENP3 was significantly higher than that of CD68^(+)CD206^(+)cells expressing SENP3.Conclusions In the nasal mucosal inflammation infiltrated with eosinophil,down-regulation of SENP3 expression in macrophages promotes the polarization of the alternative activated macrophage.

关 键 词：嗜酸性粒细胞浸润性鼻黏膜炎症 SUMO特异性蛋白酶3 巨噬细胞 

分 类 号：R765[医药卫生—耳鼻咽喉科]