hESC衍生间充质干细胞可显著促进体外血管生成  

hESC-Derived Mesenchymal Stem Cells are Superior in Promotion of Angiogenesis in Vitro

作　　者：闫永利 叶进培 YAN Yongli;YE Jinpei(Institute of Biomedical Sciences,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学生物医学研究院,太原030006

出　　处：《中国细胞生物学学报》2021年第8期1561-1573,共13页Chinese Journal of Cell Biology

摘　　要：人胚胎干细胞(hESC)是具有体外无限增殖能力和多向分化潜能的一类亚全能干细胞,在特定的条件下可被诱导分化为机体的各种细胞包括间充质干细胞(MSC)。该研究以人脂肪间充质干细胞(ADSC)和脐带间充质干细胞(UC-MSC)为对照,对hESC衍生间充质干细胞(hESC-MSC)在体外血管生成中的作用进行了系统的研究,包括其条件培养上清对脐静脉血管内皮细胞(HUVEC)的功能和向血管内皮细胞分化潜能的影响。研究发现,hESC-MSC的条件培养上清可显著促进HUVEC的增殖和迁移,其促进HUVEC增殖的作用显著高于UC-MSC的条件培养上清;hESC-MSC经不同血管内皮细胞诱导方案诱导后均具有体外类血管网络生成的能力,其网络长度均显著高于ADSC和UC-MSC经单一血管内皮细胞诱导方案诱导后的长度。因此,hESC-MSC可显著促进体外血管生成,提示该细胞有望成为一种有效的治疗新型心血管疾病的细胞。hESC(human embryonic stem cell)is a kind of pluripotent stem cells with unlimited proliferation ability and multi-lineage differentiation potential in vitro,which can be induced into a variety of cells including MSC(mesenchymal stem cell)under appropriate conditions.The present study systematically investigated the in vitro proangiogenic effect of hESC-MSC(hESC-derived mesenchymal stem cell),which was generated in this laboratory recently.The experiments included the effect of hESC-MSC’conditioned culture supernatants on the function of HUVEC(umbilical vein endothelial cell)and their potential to differentiate into vascular endothelial cells,in comparison with human ADSC(adipose-derived mesenchymal stem cell)and UC-MSC(umbilical cord mesenchymal stem cell).It was found that the CM(conditioned medium)from hESC-MSC could significantly promote the proliferation and migration of HUVEC,and the proliferation of HUVEC was significantly enhanced with the CM from hESC-MSC compared with that from UC-MSC.Moreover,the differentiating hESC-MSC could form an extensive tube-like network in vitro after induction of differentiation towards vascular endothelial cells by all protocols tested,and the length of putative tubing was significantly longer than that of differentiating ADSC and UC-MSC,from which the tube-like structure formation appeared only occasionally.In conclusion,hESC-MSC cansignificantly promote angiogenesis in vitro,suggesting that this novel source of MSC may be able to become an ideal therapeutic cell type for cardiovascular diseases.

关 键 词：人胚胎干细胞 间充质干细胞 血管生成 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]