对卵磷脂小体异常前列腺液差异蛋白的分析及慢性前列腺炎免疫机制的探讨  

Differential proteins in the prostate fluid with abnormal lecithin bodies and immunologic mechanism of chronic prostatitis patients

作　　者：张予东 刘帅 宋涛涛 金珊[1] ZHANG Yu-dong;LIU Shuai;SONG Tao-tao;JIN Shan(Department of Urology,The First Affiliated Hospital of Tianjin University of Chinese Medicine,Tianjin 300192,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300000,China)

机构地区：[1]天津中医药大学第一附属医院泌尿外科,天津300192 [2]国家中医针灸临床研究中心,天津300000

出　　处：《中华男科学杂志》2021年第7期609-615,共7页National Journal of Andrology

基　　金：天津市卫生健康委中西医结合课题(2019068)。

摘　　要：目的:研究卵磷脂小体异常时蛋白变化,探索其与慢性前列腺炎疾病病理机制的关系。方法:运用TMT技术筛选正常人群卵磷脂小体异常的前列腺液差异蛋白,收集正常人样本,规范采集前列腺液,筛选差异蛋白,使用GO功能注释和KEGG通路分析其生物学功能。结果:检测出差异蛋白377个,上调238个,下降139个,前20个显著差异蛋白与炎症免疫调控相关的有8个。GO注释富集到1 011条目,前30个条目中免疫相关功能5条,炎症反应3条。KEGG通路共有251条,与炎症免疫调控相关的9条。结论:卵磷脂小体的变化导致前列腺液蛋白组学的改变,与炎症反应和免疫失调相关蛋白和通路关系密切,可能与慢性前列腺炎免疫机制相关,可以作为慢性前列腺炎检查的一项指标。Objective: To study the protein changes in the prostate fluid with abnormal lecithin bodies, and explore their relationship with the pathogenesis of chronic prostatitis. Methods: Using the tandem mass tag(TMT) technology, we identified differential proteins in the prostate fluid with abnormal lecithin bodies in normal males and analyzed their biological functions by GO functional annotation and KEGG pathways. Results: Totally, 377 differential proteins were identified, 238 up-regulated and the other 139 down-regulated, and 8 of the top 20 significant differential proteins were related to inflammation and immune regulation. In the top 30 of the 1 011 items enriched by GO annotation, 5 were related to immune function and 3 to inflammatory response, and of the 251 KEGG pathways, 9 were involved in inflammation and immune regulation. Conclusion: Changes in lecithin bodies lead to proteomic changes in the prostatic fluid, which is closely related to protein-and pathway-associated inflammatory response and immune disorders, as well as to the immunologic mechanism of chronic prostatitis patients, and therefore can be used as an indicator of chronic prostatitis.

关 键 词：卵磷脂小体 蛋白组学 炎症反应 免疫调节 慢性前列腺炎 

分 类 号：R697.3[医药卫生—泌尿科学]