《中华人民共和国药典》收载的鹿源药材中γ-氨基丁酸含量分析  

Content Analysis of γ-aminobutyric Acid in Deer Related Chinese Herbal Materials Contained in Pharmacopoeia of The People’s Republic of China

作　　者：宫瑞泽 刘松鑫 夏蕴实 刘畅[1] 孙印石[1] 张磊[1] GONG Ruize;LIU Songxin;XIA Yunshi;LIU Chang;SUN Yinshi;ZHANG Lei(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,Jilin,China;Chinese Medicinal Materials College,Jilin Agricultural University,Changchun 130118,Jilin,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《中华中医药学刊》2021年第8期25-30,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点研发计划(2018YFC1706604,2018YFC1706605);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS);吉林省科技发展计划(20180201076YY)。

摘　　要：目的对《中华人民共和国药典》收载的鹿源药材中γ-氨基丁酸进行含量分析。方法采用柱前衍生UPLC-PDA法对不同基原、形态、采收时期,不同加工方式和部位鹿茸及鹿源药材中γ-氨基丁酸进行含量分析。结果鹿茸、鹿角、鹿角胶和鹿角霜中γ-氨基丁酸含量差异有统计学意义(P<0.05),以鹿茸(0.35-2.91 g/kg)最高,鹿角胶(0.71-0.94 g/kg)其次,鹿角和鹿角霜含量(0-0.13 g/kg)最低;不同基原鹿茸中γ-氨基丁酸含量以花鹿茸最高;不同形态鹿茸中γ-氨基丁酸含量以二杠最高,三岔其次,毛桃最低;不同生长阶段鹿茸中γ-氨基丁酸含量以二茬茸最高,激素催生二茬茸最低;不同加工方式及部位对鹿茸中γ-氨基丁酸含量影响较大,煮炸与冻干相比能较大程度保留鹿茸中的γ-氨基丁酸,单位质量排血茸中γ-氨基丁酸含量显著高于带血茸(P<0.05),蜡片、粉片、纱片和骨片中γ-氨基丁酸含量逐渐递减。结论γ-氨基丁酸在鹿源药材中广泛存在且差异显著,尤其在不同基原、形态、采收时期,不同加工方式和部位鹿茸中,可以考虑将γ-氨基丁酸作为鹿源药材的质量控制成分。Objective In order to analyze the contents of deer related Chinese herbal materials contained in Pharmacopoeia of The People’s Republic of China. Methods The pre-column derivatization UPLC-PAD method was used to analyze the content of γ-aminobutyric acid in different species, forms, harvesting periods, different processing methods and parts of Lurong(Cervi Cornu Pantotrichum) and deer related Chinese herbal materials. Results The contents of γ-aminobutyric acid in Lurong(Cervi Cornu Pantotrichum), Lujiao(Cervi Cornu), Lujiaojiao(Cervi Cornus Colla) and Lujiaoshuang(Cervi Cornu Degelatinatum) were significantly different(P<0.05). The content of γ-aminobutyric acid in Lurong(Cervi Cornu Pantotrichum)(0.35-2.91 g/kg) was the highest, that of Lujiaojiao(Cervi Cornus Colla)(0.71-0.94 g/kg) was the second, and that of Lujiao(Cervi Cornu) and Lujiaoshuang(Cervi Cornu Degelatinatum)(0-0.13 g/kg) was the lowest. The content of γ-aminobutyric acid in Hualurong(Cervus nippon Temminck) was the highest. The contents of γ-aminobutyric acid in different forms of Lurong(Cervi Cornu Pantotrichum) were different. The content in two branches was the highest, that in three branches was the second, and that in single branch was the lowest. The contents of γ-aminobutyric acid in Lurong(Cervi Cornu Pantotrichum) at different growth stages were also different. The content in Lurong(Cervi Cornu Pantotrichum) was the highest, and the content in the regrowth Lurong(Cervi Cornu Pantotrichum) produced by hormones was the lowest. Different processing methods and parts had a great influence on the content of γ-aminobutyric acid in Lurong(Cervi Cornu Pantotrichum). Compared with the freeze-drying processing method, boiling processing method can greatly preserve γ-aminobutyric acid. The content of γ-aminobutyric acid in the same quality of processed Lurong(Cervi Cornu Pantotrichum) without blood was significantly higher than that of processed Lurong(Cervi Cornu Pantotrichum) with blood(P<0.05). The content of γ-aminobutyric acid in w

关 键 词：鹿茸 鹿角 鹿角胶 鹿角霜 质量控制成分 Γ-氨基丁酸 

分 类 号：R284.1[医药卫生—中药学]