血清miR-150表达水平与脓毒症患儿疾病严重程度及预后的相关性分析  

Correlation analysis of serum miR-150 expression level with disease severity and prognosis in children with sepsis

作　　者：赵晓敏 江晓宇 单凯莉 单铁英 王洪新 张倩[1] 刘秀珍[1] ZHAO Xiaomin;JIANG Xiaoyu;SHAN Kaili;SHAN Tieying;WANG Hongxin;ZHANG Qian;LIU Xiuzhen(Department of Pediatrics,Handan Central Hospital of Hebei Province,Handan,Hebei 056000,China)

机构地区：[1]河北省邯郸市中心医院儿科,河北邯郸056000

出　　处：《国际检验医学杂志》2021年第18期2209-2213,2218,共6页International Journal of Laboratory Medicine

基　　金：河北省2019年医学科学研究课题计划(20191835)。

摘　　要：目的探讨脓毒症患儿血清miR-150表达水平与疾病严重程度、炎性反应和预后的关系。方法选取2016年1月至2018年12月该院收治的137例脓毒症患儿作为脓毒症组,同期收治的非脓毒症住院患儿87例作为对照组。检测两组患儿血清miR-150表达水平,采用Pearson相关性分析探讨miR-150表达水平与疾病严重程度和炎性反应的相关性,采用Logistic回归分析脓毒症患儿不良预后的影响因素。结果脓毒症组血清miR-150表达水平低于对照组(P<0.05)。低水平miR-150组序贯器官衰竭评估(SOFA)评分、急性生理与慢性健康状况评分系统Ⅱ(APACHEⅡ)评分以及降钙素原、C反应蛋白水平均高于高水平miR-150组,且miR-150表达水平与APACHEⅡ评分、SOFA评分、降钙素原和C反应蛋白水平呈负相关(P<0.05)。APACHEⅡ评分升高,高水平的乳酸、血肌酐、降钙素原、C反应蛋白,以及低水平的血清清蛋白和血清miR-150均是脓毒症患儿28 d死亡的危险因素(P<0.05)。结论脓毒症患儿血清miR-150表达水平降低,能够反映疾病的严重程度和炎症水平,预测患儿的死亡风险,可作为协助评估脓毒症患儿病情和预后的血清学指标。Objective To investigate the association between the expression level of miR-150 in the peripheral blood of septic children and disease severity,inflammatory reaction and outcome.Methods A total of 137 children with sepsis admitted to a hospital from January 2016 to December 2018 were selected as the sepsis group,and 87 children without sepsis hospitalized in a hospital during the same period were selected as the control group.The expression level of serum miR-150 were detected in the two groups of children.Pearson correlation analysis was used to explore the correlation between miR-150 expression level and disease severity and inflammatory response.Logistic regression was used to analyze the influencing factors of poor prognosis in children with sepsis.Results The expression level of serum miR-150 in sepsis group was lower than that in control group(P<0.05).The sequential organ failure assessment(SOFA)score,acute physiology and chronic health evaluation scoring systemⅡ(APACHEⅡ)score,procalcitonin and C-reactive protein in low-level miR-150 group were higher than that in high-level miR-150 group,the expression level of miR-150 was negatively correlated with APACHEⅡscore,SOFA score,procalcitonin and C-reactive protein(P<0.05).APACHEⅡscore increased,high level of lactate,serum creatinine,procalcitonin,C-reactive protein,low level of serum albumin and serum miR-150 were the risk factors of 28 day death in children with sepsis(P<0.05).Conclusion The expression level of miR-150 in the peripheral blood of children with sepsis is low,which can reflect disease severity and inflammatory reaction,predict the risk of death,and could be used as a serological index to evaluate the disease condition for children with sepsis.

关 键 词：miR-150 脓毒症 炎性反应 预后 

分 类 号：R459.7[医药卫生—急诊医学]