贝克曼DxH800血液分析仪出现大血小板报警信息时血小板计数结果的可靠性评价  

Reliability evaluation of platelet results when Beckmen DxH800 displays the giant platelets alarm information

作　　者：陈雅文 廖生俊[1] CHEN Yawen;LIAO Shengjun(Department of Clinical Laboratory,Zhongnan Hospital,Wuhan University,Wuhan,Hubei 430071,China)

机构地区：[1]武汉大学中南医院检验科,湖北武汉430071

出　　处：《国际检验医学杂志》2021年第18期2249-2252,2257,共5页International Journal of Laboratory Medicine

摘　　要：目的评价贝克曼DxH800血液分析仪(简称DxH800)出现大血小板报警信息时血小板计数结果的可靠性,以及如何根据血小板直方图判断大血小板对血小板计数的影响程度。方法选取2020年5—9月DxH800检测武汉大学中南医院住院部患者的血常规标本作为研究对象,当出现大血小板的报警信息时,通过比较仪器检测和人工显微镜计数结果,评价DxH800检测血小板计数结果的可靠性,并进一步分析大血小板不同占比情况下,血小板直方图的不同形态。结果101106份住院部患者的血常规标本,656例出现了大血小板的报警信息,经过人工显微镜镜检,发现其中有94例血小板计数是假性降低,3例比人工显微镜计数结果偏高。通过比较仪器检测血小板计数、人工显微镜计数的血小板计数结果,发现当大血小板所占比例>20%时,仪器检测血小板计数结果出现统计学误差,观察大血小板所占比例不同的标本的血小板直方图,均呈现“平台式”,且随着大血小板比例的升高,血小板直方图尾部与X轴间的截距越大。结论DxH800出现大血小板报警信息时血小板计数结果85.4%是可靠的,而通过观察血小板直方图的形态可大致判断大血小板的比例,并及时通过人工显微镜镜检或其他方法对血小板计数结果进行纠正,避免大血小板比例较高样本的血小板计数结果出现较大偏差。Objective To evaluate the reliability of the platelet count results when the Beckmen DxH800 displays the giant platelets alarm information,and show how to evaluate the reliability of the platelet count based on the platelet histogram.Methods The specimens tested by the DxH800 in the inpatient department of Wuhan University Zhongnan Hospital from May to September 2020 were investigated.The platelets count of the instrument and microscopy were compared when DxH800 displays the giant platelets alarm information,to evaluate the reliability of the DxH800 platelet count results,and further research on the different forms of platelet histograms.Results The total of 101106 blood samples were tested by DxH800.There were a total of 656 cases with large platelet alarm messages,of which 94 cases had a false decrease in platelet count,and 3 cases were higher than the manual count.When the proportion of giant platelets is greater than 20%,the platelet results were inaccurate and the platelet histograms all show a"platform".As the proportion of large platelets increases,the intercept between the tail of the platelet histogram and the X axis becomes larger.Conclusion When DxH800 displays the giant platelets alarm information,85.4%platelet results are reliable.The proportion of large platelets can be roughly estimated by observing the shape of the platelet histogram,so as to correct the results in time.This can effectively avoid serious deviations in the platelet counts caused by giant platelets.

关 键 词：贝克曼DxH800 报警信息 大血小板 血小板计数 血小板直方图 

分 类 号：R446.11[医药卫生—诊断学]