一款含鲜奶面霜对面部皮肤细菌多样性的影响研究  

Study on the effect of a fresh milk cream on facial skin bacterial diversity

作　　者：马雪 羡志明[2] 董强[2] 郑超 盘瑶 赵华[1] Xue Ma;Zhiming Xian;Qiang Dong;Chao Zheng;Yao Pan;Hua Zhao(Department of Cosmetics,College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China;Tianjin Yumeijing Group Co.,Ltd.,Tianjin 300110,China)

机构地区：[1]北京工商大学化学与材料工程学院化妆品系,北京100048 [2]天津郁美净集团有限公司,天津300110

出　　处：《日用化学工业》2021年第9期859-864,共6页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金资助项目(81903361);天津郁美净集团创新研究基金(YMJ20190801);北京工商大学研究生科研能力提升计划项目。

摘　　要：利用高通量测序技术研究使用一款含鲜奶面霜后对皮肤细菌多样性的影响。通过随机抽取10名使用含鲜奶面霜的受试者(女性,平均年龄24.1±0.9岁)并用棉拭子采集面颊皮肤样本,提取DNA及PCR扩增后,进行16S rRNA高通量测序,并根据97%的相似度对序列进行OTU聚类和物种分类注释,分析比较使用样品前后皮肤细菌群落组成、多样性和差异菌群。研究发现使用含鲜奶面霜后脸颊皮肤细菌的多样性降低,但受试者面颊皮肤的细菌种类及相对丰度较为相似,同时受试者皮肤菌群中的厚壁菌门和放线菌门比例明显升高,这主要是由厚壁菌门中的葡萄球菌属和放线菌门中的丙酸杆菌属比例升高导致的。结果表明此款含鲜奶面霜对皮肤菌群微生态发挥一定的调节作用,且具有滋润保湿的效果,可以作为日常护肤中的保湿产品使用。As the body’s largest organ,skin is the home of billions of microbes and helps protect the host from the external pathogens.The skin microbial barrier can be affected by endogenous factors(skin location,age,gender,and race)and exogenous factors(environment,antibiotics,and cosmetics).Cosmetics contain complex ingredients,such as preservatives,fragrances,emulsifiers,moisturizers,etc.These substances may affect the skin’s microenvironment.The purpose of this study is to investigate the effects of a cream containing fresh milk on the bacterial diversity of facial skin using high-throughput sequencing technology.A total of 10 subjects were enrolled and they were all females with the average age of 24.1 years old.The subjects used a cream containing fresh milk for 28 days,and the cheek skin microbiological specimens were collected by cotton swabs before and 28 days after the use of the fresh milk cream.DNA was extracted and amplified by PCR,and 16S rRNA high-throughput sequencing was carried out.OTU clustering and species classification annotation were performed to analyze and compare the bacterial community composition,diversity and differences of the facial skin before and after using the fresh milk cream.It is found that after using the fresh milk cream,the alpha diversity parameters including Chao1 and Shannon diversity index of the cheek skin decrease,and the distribution of the samples becomes more concentrated.The species composition at phylum level after using the cream is the same as the baseline,indicating that the use of this cream has little impact on the skin microbial health.However,the proportion of Firmicutes and Actinomycetes in the skin flora of the subjects after using the cream significantly increase,which is mainly caused by the increase of the proportion of Staphylococcus and Propionibacterium,respectively.The Staphylococcus resides in the skin area that is rich in moisture,whereas Propionibacterium plants in the area with abundant sebum content.Additionally,no skin adverse reactions are obser

关 键 词：皮肤 含鲜奶面霜 细菌多样性 高通量测序 

分 类 号：TQ658[化学工程—精细化工]