在质体中表达EPSPS赋予烟草草甘膦抗性  

Expression of an novel 5-enolpyruvylshikimate-3-phosphate synthase genes(EPSPS)in plastids conferring glyphosate resistance in tobacco

作　　者：李凡 路史展 刘羽鑫 林拥军[1] 吴高兵[2] 周菲[1] LI Fan;LU Shizhan;LIU Yuxin;LIN Yongjun;WU Gaobing;ZHOU Fei(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;College of Plant Science&Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《华中农业大学学报》2021年第5期1-8,共8页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31771752);中央高校基本科研业务费专项(2662019PY083)。

摘　　要：在烟草质体中应用来源于玉米的复合型顺式表达元件驱动来源于Isoptericola variabilis的新型耐草甘膦EPSPS基因AroA_(I.va*)的表达,使其在叶片及非绿色组织中同时高效表达。对获得的同质化质体转化植株进行转录及蛋白水平分子检测,结果表明:AroA_(I.va*)基因在转基因植株叶片及根部组织中均得到有效表达;达到同质化的烟草转基因植株pLSZ3#2和pLSZ3#3的T_(0)代种子可耐受高于12 mmol/L(2000 mg/L)的草甘膦处理,在温室中培养的转基因植株可耐受1800 mg/L剂量的草甘膦喷施处理。野生型与转基因植株的正反交实验证实通过质体转化获得的草甘膦抗性转基因植株具有母性遗传的特点。A novel glyphosate-tolerant EPSPS gene AroA_(I.va*)derived from Isoptericola variabilis n and driven by maize-derived composite cis-element was efficiently and simultaneously expressed in the plastids of leaves and non-green tissues of tobacco.The results of transcription and protein-level determination of the homoplastomic transplastomic lines showed that the AroA_(I.va*)gene was effectively expressed in the leaf and root tissues.The T_(0) seeds of homoplastomic transplastomic plants pLSZ3#2 and pLSZ3#3 tolerated more than 12 mmol/L glyphosate treatment.The transgenic plants cultivated in the greenhouse tolerated the spraying treatment with glyphosate at a dose of 1800 mg/L.Reciprocal crosses between wild-type and transgenic plants confirmed that the glyphosate-resistant transgenic plants obtained by plastid transformation had the characteristics of maternal inheritance.

关 键 词：质体转化 烟草 AroA I.va* 草甘膦 5-烯醇式丙酮酰莽草酸-3-磷酸合酶 转基因 母系遗传 

分 类 号：Q786[生物学—分子生物学]