湖北枫杨水煎液诱导CIA模型大鼠滑膜细胞凋亡机制研究  

Mechanism of Hubei Fengyang Decoction Induced-Apoptosis of Synovial Cells in CIA Rats

作　　者：陈明 向阳 CHEN Ming;XIANG Yang(Department of Medicine,Hubei University for Nationalities,Enshi 445000,China;HubeiProvinceKey Laboratory of occurrence and intervention of RheumaticDiseases(Hubei University for Nationalities),Enshi 445000,China)

机构地区：[1]湖北民族大学医学部,湖北恩施445000 [2]风湿病发生与干预湖北省重点实验室(湖北民族大学),湖北恩施445000

出　　处：《中国民族民间医药》2021年第17期23-29,共7页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金资助项目(No.81260458,No.81360672)。

摘　　要：目的:探究枫杨诱导类风湿关节炎大鼠细胞凋亡的机制,为枫杨治疗类风湿关节炎提供科学依据。方法:雄性健康SD大鼠60只,随机分为空白组、模型组、雷公藤多苷组、枫杨水煎液低、中、高剂量组,除空白组外,其余各组建立CIA模型。造模成功后,分别给予相应治疗,测量膝关节直径,进行关节炎评分,观察滑膜切片,检测血清炎症因子水平和相关凋亡蛋白的表达。结果:①一般情况:与模型组比较,各用药组体重明显增加,膝关节直径、关节炎指数均有所下降,其中枫杨高剂量组效果优于雷公藤多苷组(P<0.05)。②滑膜组织病理形态观察:HE染色结果显示,模型组大鼠膝关节有大量炎性细胞浸润。枫杨治疗组能够显著缓解膝关节肿胀程度,抑制足部的红肿,HE染色镜下表现及评分也有所改善(P<0.05)。③细胞因子和相关蛋白:与模型组相比,各治疗组TGF-β、IL-4水平明显升高,IL-17A、IFN-γ水平显著降低(P<0.05);滑膜组织中,Bcl-xl蛋白的表达降低,Caspase-8表达增加(P<0.05)。对于TNFRII,则存在枫杨低剂量组降低,中、高剂量组明显上调其表达(P<0.05)。结论:枫杨水煎液可有效缓解CIA大鼠临床症状及病理改变,降低血清炎性细胞因子、提升抗炎因子水平,上调滑膜促凋亡蛋白、下调凋亡抑制蛋白表达,促进CIA大鼠滑膜细胞凋亡。Objective To observe the therapeutic efficacy of Fengyang on rheumatoid arthritis and evaluate the effect of Fengyang to induce synovial fibroblast cell apoptosis in collagen-induced arthritic(CIA)rats.Methods Sixty healthy male SD rats divided into CIA control control(CC)group,Triptery giumpoly glycoside(TG)-treating group,and three Fengyang-treating(FY)groups bred with low,medium and high dose of Fengyang decoction.After successful establishment of the model,the diameter of knee joint was measured,the score of arthritis was scored,the synovium section was observed,and the level of serum inflammatory factors and the expression of apoptosis-related proteins were detected.Results①General conditions:Compared with the model group,the weight of rats in each medication group increased significantly,the diameter of knee joint and the arthritis index decreased,among which,the effect of the high dose group of FY was better than that of the TG group(P<0.05).②Pathological observation of synovial tissue:The results of HE staining showed that there was a large amount of inflammatory cell infiltration in the knee joints of rats in the model group.FY groups could significantly alleviate the swelling degre of knee joint,inhibit the swelling of foot,and decrease the score of HE staining microscopy(P<0.05).③ELISA results for cytokines and Western Blot test for apoptosis-associated protein:Compared with the model group,the levels of TGF-βand IL-4 were significantly increased and the levels of IL-17A and IFN-γwere significantly decreased in each treatment group(P<0.05).④Western Blot test for apoptosis-associated proteins:Compared with the CC group,the expression of TNFRII in medium-dose and high-dose FY groups was significantly increased(P<0.05);The expression of Caspase-8 protein in each drug group was significantly increased(P<0.05);The expression of Bcl-xl protein was decreased in all medication groups(P<0.05).Conclusion The efficacy of Fengyang in treatment of collagen-induced arthritic rats may partially obtained by

关 键 词：枫杨 胶原诱导关节炎 滑膜细胞 凋亡 

分 类 号：R593.22[医药卫生—内科学]