NGS-metabarcoding revealing novel foraminiferal diversity in the Western Pacific Magellan Seamount sediments  

作　　者：Junfeng SHI Yanli LEI Haotian LI Tiegang LI 

机构地区：[1]Laboratory of Marine Organism Taxonomy and Phylogeny,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Key Laboratory of Marine Sedimentology and Environmental Geology,First Institute of Oceanography,Ministry of Natural Resources(MNR),Qingdao 266061,China [3]Clinical Research Center,Affi liated Hospital of Weifang Medical University,Weifang 261031,China [4]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519082,China [5]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [6]University of Chinese Academy of Sciences,Beijing 100049,China [7]Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China

出　　处：《Journal of Oceanology and Limnology》2021年第5期1718-1729,共12页海洋湖沼学报（英文）

基　　金：the Science&Technology Basic Resources Investigation Program of China(No.2017FY100804);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42000000);the National Program on Global Change and Air-Sea Interaction(No.GASIGEOGE-04);the Key Program of National Natural Science Foundation of China(No.41930533);the Taishan Scholars Project Funding(No.TS20190963);the Senior User Project of R/V Kexue;Center for Ocean Mega-Science,Chinese Academy of Sciences;。

摘　　要：Seamount is a unique deep-sea ecosystem widely distributed in the world.Its biodiversity is vibrant due to its specific geographical and hydrological conditions.However,the diversity and features of foraminifera in such an environment have rarely been studied.We extracted environmental DNA(eDNA)in sedime nts and amplified the partial small subunit ribo somal DNA(SSU rDNA)of fo raminife ra to understand the foraminiferal diversity from four sites in Magellan Seamount(Western Pacific Ocean).Partial S SU rDNA sequencing was conducted and 912979 foraminiferal reads were obtained and gathered into 266 operational taxonomic units(OTUs).In the available dataset,a high proportion of rare OTUs and low identity OTUs in each studied sample showed that the Magellan Seamount foraminiferal community might have a high genetic novelty.The relative abundance of foraminifera varied between replicates probably due to the existence of bias in amplification process and patchiness of the deep-sea floor.It showed that the Magellan Seamount has a relatively high benthic foraminiferal diversity characterized mainly by monothalamiids(76.37%of total reads)in association with rotaliids(19.03%of total reads),including planktic foraminiferal sequences(38.58%of rotaliids;7.36%of total reads).The remaining reads were assigned to miliolids(0.83%of total reads)and textulariids(0.66%of total reads),and 3.11%of total reads are unassigned to a specific family.The co mparative analysis with foraminiferal assemblage s from coastal and deep-sea environme nts indicated that seamounts could aggregate species from a nearby deep-sea.

关 键 词：metabarcoding Magellan Seamount FORAMINIFERA Western Pacific Ocean 

分 类 号：Q958.8[生物学—动物学]