鸭疫里默氏杆菌recA基因突变对其生长及DNA损伤反应的影响  

Effects of recA Gene Mutation on the Growth and DNA Damage Response of Riemerella anatipestifer

作　　者：吉果 陈启伟[1] 宫晓炜[1] 刘永生[1] 郑福英[1] JI Guo;CHEN Qiwei;GONG Xiaowei;LIU Yongsheng;ZHENG Fuying(State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China)

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,兰州730046

出　　处：《畜牧兽医学报》2021年第9期2553-2560,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金面上项目(31572532)。

摘　　要：DNA损伤反应(SOS response)是细菌应对基因组DNA损伤的一种重要的保护机制。RecA蛋白在许多细菌中对SOS反应的诱导都起关键调控作用,其同源蛋白广泛存在于各种生物体中。本研究对鸭疫里默氏杆菌RecA蛋白是否调控其SOS反应进行探索。构建recA基因缺失株和回复株,检测亲本株、缺失株和回复株在紫外线辐照损伤下的生长能力、recA基因的转录水平以及SOS反应。结果表明,成功构建了recA基因缺失株ΔrecA和回复株cΔrecA;recA基因灭活对鸭疫里默氏杆菌RA-GD株的生长速率无明显影响;在紫外线辐照损伤下,亲本株recA基因的转录水平明显升高,缺失株的存活能力及其基因组的完整性都显著低于亲本株和回复株。本研究证实RecA蛋白参与鸭疫里默氏杆菌的SOS反应。DNA damage response(SOS response)refers to an important protective mechanism for bacteria to cope with genomic DNA damage.The RecA protein plays a key regulatory role in the induction of SOS responses in many bacteria,and its homologous proteins are widely present in various organisms.The purpose of this study was to explore whether RecA protein regulates the SOS response of Riemerella anatipestifer.We constructed the recA deletion mutant and complemented strain.The growth abilities of the parental strain,deletion mutant and complemented strain,the transcriptional levels of recA gene and SOS response of the above strains with ultraviolet treatment were detected.The results showed that the recA deletion mutantΔrecA and complemented strain cΔrecA were successfully constructed.Inactivation of recA gene had no significant effect on the growth rate of RA-GD strain.Under ultraviolet radiation irradiation,the transcription level of recA gene of parental strain increased significantly.Compared with the parent strain and the complemented strain,the survival ability and the genomic integrity of the deletion strain reduced significantly.This study confirmed for the first time that the RecA protein is involved in the SOS response of Riemerella anatipestifer.

关 键 词：鸭疫里默氏杆菌 RECA基因 生长 SOS反应 

分 类 号：S852.61[农业科学—基础兽医学]