金星无核与其短节间突变体紫金早生的节间长度差异机制初探  

Preliminary analysis on the mechanism leading to difference in internode length between Venus Seedless grapevine and its shorter internode mutant Zijinzaosheng

作　　者：王博 王壮伟[1] 王西成[1] 钱亚明[1] 吴伟民[1] WANG Bo;WANG Zhuangwei;WANG Xicheng;QIAN Yaming;WU Weimin(Institute of Pomology,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,Jiangsu,China)

机构地区：[1]江苏省农业科学院果树研究所·江苏省高效园艺作物遗传改良重点实验室,南京210014

出　　处：《果树学报》2021年第9期1423-1434,共12页Journal of Fruit Science

基　　金：江苏省农业科技自主创新资金[CX(20)2022];财政部和农业农村部:国家现代农业产业技术体系资助。

摘　　要：【目的】探索金星无核短节间突变体紫金早生节间长度短缩的形成机制。【方法】以金星无核(JXWH)及其短节间突变体紫金早生(ZJZS)为研究材料,借助扫描电镜、LC-MS/MS和高通量测序分析两种材料在细胞形态、内源激素含量和miRNA上的差异。调查外源激素处理(JA及其合成抑制剂DIECA)下突变体节间长度的变化。【结果】紫金早生节间长度和节间细胞数量显著低于金星无核;紫金早生中JA、JA-ILE和SA含量显著提高,而ICA和ICAld含量显著降低;小RNA测序结果发现2种材料中共有21个差异miRNA,GO注释分析发现它们主要参与细胞分裂、茉莉酸代谢、蛋白代谢、环境应答和器官发育等过程。外源DIECA处理后,紫金早生节间伸长,外源JA处理抑制了紫金早生节间长度的伸长。【结论】紫金早生短节间突变性状与茉莉酸信号通路的调节有关,茉莉酸可能受miR319的调控。【Objective】Grape industry is labor-intensive,especially in the management of new shoots.In practice,grape with shorter internode length has such unique advantages as labor saving and simplifica-tion in canopy management.Although the dwarfing mechanisms in many fruit trees have been studied,little has been studied in grapevine.The mechanism of short internode in grapevine remains unclear.Mutant is ideal materials for study the genetic mechanism of traits.However,short internode mutants of grapevine are scarce.A grape mutant named Zijinzaosheng with short internode was discovered from the progeny of Venus Seedless grapevines after treatment with colchicine.A previous study showed that the chromosome ploidy of Zijinzaosheng was the same as that of Venus Seedless.The DNA sequences had little difference between Zijinzaosheng and Venus Seedless,and the genetic background of the two materials is highly consistent.The short internode trait of Zijinzaosheng was stable in multi-plot trials for several years.The fruit of Zijinzaosheng was not sensitive to exogenous GA3.We speculated that the mutation mechanism of Zijinzaosheng was different from that of Point Meunier.In order to elucidate the mechanism and regulation of grapevine internode,comparative analyses on internode length,cell morphology of stem,endogenous hormone contents and small RNA sequencing were conducted in the study.Meanwhile,the influence of exogenous hormones on the internode length in Zijinzaosheng was also evaluated.【Methods】7-year-old trees of Venus Seedless and Zijinzaosheng were used as the experi-mental materials.They were planted in Lishui plant science station of Jiangsu Academy of Agricultural Sciences,a core demonstration vineyard of Nanjing Experimental Station of China Agriculture Re-search System for Grape Industry.All the plant materials were maintained under rain-shelter.The study was conducted in 2019,and shoot tips were collected for high throughput sequencing;the young leaves at the shoot tip were sampled to determine the endogen

关 键 词：葡萄 突变体 节间长度 MIRNA 茉莉酸 

分 类 号：S663.1[农业科学—果树学]