大麻素2型受体在七氟烷后处理减轻大鼠肠缺血再灌注损伤中的作用  

Role of cannabinoid type 2 receptor in sevoflurane postconditioning-induced reduction of intestinal ischemia-reperfusion injury in rats

作　　者：叶立 陈萌 季拓 张宗泽[1] 何祥虎[1] Ye Li;Chen Meng;Ji Tuo;Zhang Zongze;He Xianghu(Department of Anesthesiology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院麻醉科,430071

出　　处：《中华麻醉学杂志》2021年第5期603-606,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81000849);武汉大学中南医院科技创新培育基金(znpy2018022)。

摘　　要：目的:评价大麻素2型受体(CB2R)在七氟烷后处理减轻大鼠肠缺血再灌注损伤中的作用。方法:清洁级健康雄性SD大鼠24只,8~10周龄,体重220~270 g,采用随机数字表法分为4组(n=6):假手术组(Sham组)、肠缺血再灌注组(I/R组)、肠缺血再灌注+七氟烷后处理组(Sevo组)和肠缺血再灌注+七氟烷后处理+CB2R拮抗剂AM630组(AM组)。采用夹闭肠系膜上动脉45 min,再灌注2 h的方法制备大鼠肠缺血再灌注损伤模型。Sevo组再灌注即刻吸入2%七氟烷,持续30 min,AM组缺血前1 h腹腔注射CB2R拮抗剂AM6303 mg/kg,其余操作同Sevo组。于再灌注2 h时,麻醉后处死大鼠取小肠组织,光镜下观察肠组织病理学结果并行Chui评分,确定湿重/干重(W/D)比值,采用硫代巴比妥酸比色分析法检测MDA含量,采用髓过氧化物酶法测定MPO活性,采用Western blot法检测cleaved caspase-3的表达。结果:与Sham组比较,I/R组小肠组织Chui评分、W/D比值、MDA含量和MPO活性升高,cleaved caspase-3表达上调(P<0.05);与I/R组比较,Sevo组小肠组织Chui评分、W/D比值、MDA含量和MPO活性降低,cleaved caspase-3表达下调(P<0.05);与Sevo组比较,AM组小肠组织Chui评分、W/D比值、MDA含量和MPO活性增加,cleaved caspase-3表达上调(P<0.05)。结论:CB2R参与了七氟烷后处理减轻大鼠肠缺血再灌注损伤的过程。Objective To evaluate the role of cannabinoid type 2 receptor(CB2R)in sevoflurane postconditioning-induced reduction of intestinal ischemia-reperfusion(I/R)injury in rats.Methods Twenty-four clean-grade healthy male Sprague-Dawley rats,aged 8-10 weeks,weighing 220-270 g,were divided into 4 groups(n=6 each)using a random number table method:sham operation group(group Sham),intestinal I/R group(group I/R),intestinal I/R+sevoflurane postconditioning group(group Sevo)and intestinal I/R+sevoflurane postconditioning+CB2R antagonist AM630 group(group AM).The model of intestinal I/R injury was established by occluding the superior mesenteric artery for 45 min followed by 2 h reperfusion.In the group Sevo,2%sevoflurane was inhaled immediately at the beginning of reperfusion for 30 min.In the group AM,CB2R antagonist AM6303 mg/kg was intraperitoneally injected at 1 h before ischemia,and the other treatments were similar to those previously described in group Sevo.At 2 h of reperfusion,the animals were sacrificed after anesthesia,and small intestinal tissues were obtained for microscopic examination of the pathologic changes which was scored according to Chiu and for determination of wet/dry weight ratio(W/D ratio),for detection of malondialdehyde(MDA)content(by thiobarbituric acid colorimetry),for determination of myeloperoxidase(MPO)activity(by MPO assay)and cleaved caspase-3 protein expression(by Western blot).Results Compared with group Sham,the Chui score,W/D ratio,MDA content and MPO activity in the intestinal tissues were significantly increased,cleaved caspase-3 expression was up-regulated in group I/R(P<0.05).Compared with group I/R,the Chui score,W/D ratio,MDA content and MPO activity in the intestinal tissues were significantly decreased,cleaved caspase-3 expression was down-regulated in group Sevo(P<0.05).Compared with group Sevo,the Chui score,W/D ratio,MDA content and MPO activity were significantly increased,cleaved caspase-3 expression was up-regulated in group AM(P<0.05).Conclusion CB2R is involved in the p

关 键 词：受体 大麻酚 CB2 麻醉药 吸入 肠 再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]