鼻窦炎模型兔的Notch信号通路功能的动态变化  

Dynamic changes of Notch signaling pathway function in sinusitis model rabbits

作　　者：杨志超[1] 王丽欣[2] 梁鹏 马会梅 于洋[1] Yang Zhichao;Wang Lixin;Liang Peng;Ma Huimei;Yu Yang(General Hospital of Ningxia Medical University,Ningxia Yinchuan 750001;The People's Hospital of Shiyan City,Hubei,Hubei Shiyan 442000;Ningxia Medical University,Ningxia Yinchuan 750001)

机构地区：[1]宁夏医科大学总医院,宁夏银川750001 [2]湖北十堰市人民医院,湖北十堰442000 [3]宁夏医科大学,宁夏银川750001

出　　处：《中国社区医师》2021年第27期5-6,共2页Chinese Community Doctors

基　　金：宁夏自然科学基金,编号:2020AAC03409。

摘　　要：目的:通过构建体内外骨质重塑模型,探讨Notch信号通路与miRNA-155的相互作用,明确其在难治性鼻窦炎骨质重塑中的作用及机制。方法:选取兔鼻窦炎模型鼻窦骨质,检测Notch的表达及与骨质重塑相关细胞因子的关系。检查制备兔鼻窦炎模型,采用CT扫描及病理切片进行骨质重塑的评分。HE、碱性磷酸酶和抗酒石酸酸性磷酸酶染色,从组织学水平对标本骨质的炎症程度进行评估,探讨不同时间兔鼻窦炎症变化程度,通过CT值评估骨质重塑的程度。结果:Notch基因在慢性鼻窦炎小鼠鼻中隔黏膜中高表达,说明Notch基因在慢性鼻窦炎的炎症过程中发挥一定作用。结论:Notch信号传导通路在鼻窦炎模型兔疾病的发生发展中,具有重要的调控作用。Objective:To investigate the interaction between Notch signaling pathway and miRNA-155 by constructing a bone remodeling model in vivo and in vitro,and to clarify its role and mechanism in bone remodeling in refractory sinusitis.Methods:We selected rabbit sinusitis model sinus bone to detect the expression of Notch and its relationship with bone remodeling-related cytokines.The rabbit model of sinusitis was prepared by inspection and CT scan and pathological section were used to score bone remodeling.HE,alkaline phosphatase and tartrate resistant acid phosphatase staining were used to evaluate the degree of bone inflammation from the histological level,to explore the changes of sinusitis in rabbits at different times,and to evaluate the degree of bone remodeling by CT value.In vivo experiments,research on Notch gene and factors related to bone remodeling.Results:The Notch gene was highly expressed in the mucosa of the nasal septum in mice with chronic sinusitis,indicating that the Notch gene played a certain role in the inflammation process of chronic sinusitis.Conclusion:Notch signaling pathway plays an important regulatory role in the occurrence and development of sinusitis model rabbits.

关 键 词：鼻窦炎 模型兔 NOTCH 信号通路 

分 类 号：R765.41[医药卫生—耳鼻咽喉科] R-332[医药卫生—临床医学]