电针预处理对脓毒症相关性脑病大鼠海马氧化应激反应的影响及其与Nrf2/HO-1信号通路的关系  

Effect of electroacupuncture pretreatment on oxidative stress response of hippocampus in rats with sepsis-associated encephalopathy and relationship with Nrf2/HO-1 signaling pathway

作　　者：李翠[1] 宫丽荣[1] 武丽娜[1] 穆蕊[1] 余剑波[1] Li Cui;Gong Lirong;Wu Lina;Mu Rui;Yu Jianbo(Deportment of Anesthesiology and Critical Care Medicine,Tianjin Nankai Hospital,Tianjin 300100,China)

机构地区：[1]天津市南开医院麻醉科和重症医学科,300100

出　　处：《中华麻醉学杂志》2021年第6期750-753,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年项目(81903941);天津市科技计划项目(20JCZDJC00480)。

摘　　要：目的:评价电针预处理对脓毒症相关性脑病大鼠海马氧化应激反应的影响及其与核因子E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)信号通路的关系。方法:健康成年雄性SD大鼠64只,体重250~300 g,采用随机数字表法分为4组(n=16):假手术组(Sham组)、脓毒症相关性脑病组(SAE组)、脓毒症相关性脑病+电针组(SAE+EA组)和脓毒症相关性脑病+假电针组(SAE+SEA组)。SAE+EA组选取百会、曲池和足三里穴进行30 min/d连续5 d的电刺激。最后1次电针结束后即刻,采用盲肠结扎穿孔法建立脓毒症模型。造模后1和7 d时采用ELISA法检测海马MDA和SOD水平,采用RT-PCR法检测海马Nrf2 mRNA表达水平,采用Western blot法检测Nrf2和HO-1表达。造模后3~7 d时行Morris水迷宫实验检测大鼠认知功能,记录逃避潜伏期和目标象限探索时间。结果:与Sham组比较,SAE组造模后1和7 d时海马MDA含量增加,SOD活性降低,造模后7 d时海马Nrf2及其mRNA和HO-1表达下调,逃避潜伏期延长,目标平台探索时间缩短(P<0.05);与SAE组比较,SAE+EA组造模后1和7 d时海马MDA含量降低,SOD活性升高,Nrf2及其mRNA和HO-1表达上调,逃避潜伏期缩短,目标平台探索时间延长(P<0.05),SAE+SEA组上述指标差异无统计学意义(P>0.05)。结论:电针预处理可减轻脓毒症相关性脑病大鼠海马氧化应激反应,机制可能与激活Nrf2/HO-1信号通路有关。Objective To evaluate the effect of electroacupuncture(EA)pretreatment on oxidative stress response of hippocampus in rats with sepsis-associated encephalopathy(SAE)and the relationship with nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(Nrf2/HO-1)signaling pathway.Methods A total of 64 healthy adult male Sprague-Dawley rats,weighing 250-300 g,were divided into 4 groups(n=16 each)using a random number table method:sham operation group(Sham group),SAE group,SAE+EA group and SAE plus sham EA group(SAE+SEA group).In SAE+EA group,Baihui,Quchi and Zusanli acupoints were stimulated for 30 min once a day for 5 consecutive days.Sepsis was induced by cecal ligation and puncture immediately after the end of the last EA.At 1 and 7 days after establishment of the model,the hippocampal malondialdehyde(MDA)and superoxide dismutase(SOD)levels were detected by enzyme-linked immunosorbent assay,the expression of hippocampal Nrf2 mRNA was detected using real-time reverse transcription polymerase chain reaction,and the expression of Nrf2 and HO-1 was determined by Western blot.At 3-7 days after establishment of the model,cognitive function was assessed using the Morris water maze test,and the escape latency and the target quadrant exploration time were recorded.Results Compared with Sham group,the content of MDA was significantly increased and the activity of SOD was decreased at 1 and 7 days after establishment of the model,the expression of Nrf2 protein and mRNA and HO-1 was down-regulated at day 7 after establishment of the model,the escape latency was prolonged,and the target quadrant exploration time was shortened in SAE group(P<0.05).Compared with SAE group,the content of MDA was significantly decreased and the activity of SOD was increased at 1 and 7 days after establishment of the model,the expression of Nrf2 protein and mRNA and HO-1 was up-regulated at day 7 after establishment of the model,the escape latency was shortened,and the target quadrant exploration time was prolonged in group SAE+EA(P<0.05),and no sig

关 键 词：电刺激疗法 脓毒症相关性脑病 氧化性应激 核因子E2相关因子2 血红素加氧酶-1 

分 类 号：R245.97[医药卫生—针灸推拿学]