贵州产细毡毛忍冬中绿原酸的含量测定研究  

Determination of chlorogenic acid in Lonicera similes Hemsl.from Guizhou

作　　者：覃育往 龙滢 QIN Yuwang;LONG Ying(Libo maternal and Child Health Hospital,Libo 558499,China;Qiandongnan Inspection&Testing Center of Food and Drug,Kaili 556000,China)

机构地区：[1]荔波县妇幼保健院,贵州荔波558499 [2]黔东南州食品药品检验检测中心,贵州凯里556000

出　　处：《贵州科学》2021年第3期55-58,共4页Guizhou Science

摘　　要：目的:对贵州产细毡毛忍冬中绿原酸的含量测定研究。方法:采用高效液相色谱法,色谱柱为Thermo Accucore XL C_(18)(5μm,4.6×250 mm),流动相为乙腈-0.4%磷酸溶液(8∶92),检测波长327 nm,柱温为室温,流速1.0 mL/min,进样体积10μL。结果:绿原酸在4.7376~94.7532 mg/mL浓度范围内呈现良好的线性关系,线性回归方程为Y=0.5304 X-0.194,R^(2)=0.9999,平均回收率95.16%,RSD为0.36%,重复性RSD为0.24%,稳定性RSD为0.79%,精密度RSD为0.06%。结论:本方法操作简便,准确,精密度、重现性、稳定性、耐用性良好,可用于细毡毛忍冬中绿原酸的含量测定。In this study we determined the content of chlorogenic acid in Lonicera similes Hemsl.from Guizhou by HPLC.The column was Thermo Accucore XL C_(18)(5μm,4.6×250 mm),the mobile phase was acetonitrile-0.4%phosphoric acid solution(8∶92),the detection wavelength was 327 nm,the column temperature was room temperature,the flow rate was 1.0 mL/min,and the injection volume was 10μL.The results showed that chlorogenic acid had good linear relationship within the concentration range of 4.737694 mg/mL to 94.7532 mg/mL.The linear regression equation was Y=0.5304 X-0.194.R^(2)=0.9999.The average recovery rate was 95.16%,the RSD was 0.36%,the RSD of repeatability,stability and precision was 0.24%,0.79%,and 0.06%,respectively.This method was simple and accurate,with good precision,repeatability,stability and durability,which could be used to determine the content of chlorogenic acid in Lonicera similes Hemsl.

关 键 词：细毡毛忍冬 绿原酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]