Sly-miR166b及其靶基因在番茄抗晚疫病中的作用初探  

Preliminary Study on the Role of sly-miR166b and Its Target Genes in Tomato Resistance to Late Blight

作　　者：张晓艺 洪雨慧 张媛媛[1] 栾雨时[1] ZHANG Xiaoyi;HONG Yuhui;ZHANG Yuanyuan;LUAN Yushi(School of Bioengineering,Dalian University of Technology,Dalian,Liaoning 116024,China)

机构地区：[1]大连理工大学生物工程学院,辽宁大连116024

出　　处：《园艺学报》2021年第8期1595-1604,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31872116);大连理工大学大学生创新创业项目(2020101410101010281)。

摘　　要：为探明sly-miR166b在番茄抗晚疫病中的作用,分别构建了sly-miR166b的过表达载体和沉默载体,将它们在番茄离体叶片中瞬时表达,结果发现,过表达sly-miR166b下调了其靶基因的表达量,与空载体对照相比,晚疫病菌接种后叶片上相对病斑面积明显变小;而沉默sly-miR166b则上调了其靶基因的表达量,病菌接种后叶片上相对病斑面积较对照明显变大。初步表明,过表达sly-miR166b增强了植株对晚疫病的抗性,沉默sly-miR166b增强了植株的感病性,这为全面揭示sly-miR166b的抗病机制奠定了基础。To investigate the function of sly-miR166 b in tomato resistance to late blight,the vectors that overexpressing and silencing sly-miR166 b were constructed and transiently expressed in tomato plants respectively.The results showed that transient overexpressing sly-miR166 b(TO)reduced its target genes expression.The ratio of lesion area to leaf area on TO leaves was much lower than that in control leaves upon inoculation.On the contrary,transient silencing sly-miR166 b(TS)increased its target genes expressions.The ratio of lesion area to leaf area on TS leaves was much higher than that in control leaves upon inoculation.This study preliminarily showed that overexpression of sly-miR166 b enhanced plant resistance to late blight,while silencing sly-miR166 b enhanced plant susceptibility to late blight,laying a foundation for comprehensively revealing the sly-miR166 b mechanism in disease resistance.

关 键 词：番茄 晚疫病 MIRNA 靶基因 抗病 

分 类 号：S641.2[农业科学—蔬菜学]