溶胶-凝胶分子印迹CsPbBr_(3)量子点的构建及其在胆固醇荧光检测中的应用  

Construction of a Sol-Gel Molecularly Imprinted CsPbBr_(3) Quantum Dots Probe and Its Application in Fluorescence Detection of Cholesterol

作　　者：宋敏霞 管杰 陈璐 舒韵 徐琴[1] 胡效亚[1] SONG Min-xia;GUAN Jie;CHEN Lu;SHU Yun;XU Qin;HU Xiao-ya(School of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002,China)

机构地区：[1]扬州大学化学化工学院,江苏扬州225002

出　　处：《分析测试学报》2021年第9期1367-1373,共7页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(NSFC22076161,21675140,21705141)。

摘　　要：该文研究了CsPbBr_(3)量子点(QDs)作为一种新型荧光(FL)探针对胆固醇(CHO)的检测。以3-氨丙基三乙氧基硅烷(APTES)为功能单体,CHO为模板分子,采用溶胶-凝胶技术,将CHO引入到SiO_(2)网络中,制备了CHO分子印迹膜保护的CsPbBr_(3)@SiO_(2)QDs。该分子印迹膜既可降低空气、湿度等与CsPbBr_(3)QDs的接触,提高CsPbBr_(3)QDs的稳定性,又赋予CsPbBr_(3)QDs对CHO的选择性。检测过程中,CHO被捕获到CsPbBr_(3)@SiO_(2)QDs的印迹位点上,形成空间位阻,导致CsPbBr_(3)QDs荧光强度下降。在最优条件下,CsPbBr_(3)QDs的荧光猝灭效率和CHO浓度对数在1.00×10^(-11)~5.00×10^(−8)mol·L^(-1)范围内呈现良好的线性关系,检出限为2.48×10^(-12)mol·L^(-1)。结果表明,该方法灵敏度高、选择性好。该工作为CHO检测提供了一种新的策略,拓宽了CsPbBr_(3)QDs的应用范围。In this work,CsPbBr_(3) quantum dots(QDs)were used as a new kind of fluorescence(FL)probe to detect cholesterol(CHO).By using 3-aminopropyltrimethoxysilane(APTES)as the functional monomer and CHO as the template molecule,a CHO molecularly imprinted layer was formed on CsPbBr_(3) QDs via a simple silica sol-gel process.The formed silica layer not only improve the stability of CsPbBr_(3) QDs by protecting CsPbBr_(3) QDs from direct exposure to air and humidity,but also renders CsPbBr_(3) QDs good selectivity to CHO.During the detection process,CHO was captured into the specific cavity sites of CHO-imprinted SiO_(2) shell layers on CsPbBr_(3)@SiO_(2) QDs to cause the decrease of the FL intensity of CsPbBr_(3) QDs.Under the optimal conditions,a good linear relationship was obtained between fluorescence quenching efficiency and the logarithm of CHO concentrations in the range of 1.00×10^(-11)-5.00×10^(-8) mol·L^(-1),with a detection limit down to 2.48×10^(-12) mol·L^(-1).This sensor showed better sensitivity and selectivity to CHO than to other interferences.This work broadens the application horizon of CsPbBr_(3) QDs and provides a new strategy for CHO detection.

关 键 词：溶胶-凝胶 钙钛矿量子点 胆固醇 荧光传感器 

分 类 号：O657.3[理学—分析化学]