蝉拟青霉产镇痛组分的培养条件优化  

Optimization of culture conditions for analgesic components produced by Paecilomyces cicadae

作　　者：罗钰嘉 陈波利 谭艾娟 吕世明[2] 孙海峰 艾仁丽 LUO Yu-jia;CHEN Bo-li;TAN Ai-juan;LYU Shi-ming;SUN Hai-feng;AI Ren-li(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China;College of Animal Sciences,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院山地植物资源保护与保护种质创新教育部重点实验室山地生态与农业生物工程协同创新中心,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《生物技术》2021年第4期377-384,共8页Biotechnology

基　　金：国家自然科学基金项目(31760749);贵州省科技计划项目(黔科合平台人才[2017]5788号)。

摘　　要：[目的]研究蝉拟青霉产镇痛组分的最佳液体发酵培养条件。[方法]采用Plackett-Burman试验设计对液体培养基中葡萄糖、蛋白胨、酵母浸膏、KH2PO4和MgSO4以及发酵过程中p H、温度和接种量8个因素进行变量分析,筛选出了重要的影响因子为培养基p H、发酵温度和接种量;通过最陡爬坡试验得到这些因子的最大响应值区域。[结果]应用响应面方法分析得到最佳培养条件为:葡萄糖20 g/L、蛋白胨3 g/L、酵母浸膏2 g/L、KH2PO41 g/L、MgSO40.5 g/L、p H 7.96、温度16.05℃及接种量9.27%。[结论]优化后得到的镇痛组分含量是原条件的7.633倍,为该菌株镇痛组分的工业化发酵生产奠定了较好的基础。[Objective]To study the optimal culture conditions for extracting analgesic components from the liquid fermentation of Paecilomyces cicadae.[Method]the eight-variable analysis of glucose,peptone,yeast extract,KH2 PO4,MgSO4,p H,temperature,and inoculum were analyzed with the Plackett-Burman test design,and the important factors,such as p H,temperature,and inoculum were selected out from 8 factors.Then the area of the maximum response value of factors were obtained through the steepest climbing test,and the maximum response value of factors were analyzed by the response surfaces method.[Result]The optimal culture conditions were glucose 20 g/L,peptone 3 g/L,yeast extract 2 g/L,KH2 PO41 g/L,MgSO40.5 g/L,p H 7.96,temperature 16.05℃and inoculation volume 9.27%.[Conclusion]The content of analgesic components obtained after optimization is 7.633 times of the original conditions.After optimization,it adds a better foundation for the industrialized fermentation production of the baseline analgesic component.

关 键 词：蝉拟青霉 镇痛 液体培养 响应面 

分 类 号：Q512[生物学—生物化学]