车前子对腹泻大鼠小肠组织AQP3基因及蛋白表达的影响  

Effect of Semen Plantaginis on Expression of AQP3 Gene and Protein in Small Intestine of Diarrhea Rats

作　　者：张睦清[1] 彪雅宁 李易水 张纳博 韩雪 李博姸 张一昕 ZHANG Muqing;BIAO Yaning;LI Yishui;ZHANG Nabo;HAN Xue;LI Boyan;ZHANG Yixin

机构地区：[1]河北省中医院心内科,河北石家庄050011 [2]河北中医学院药学院临床中药学教研室(河北省高校中药组方制剂应用技术研究中心),河北石家庄050200

出　　处：《新中医》2021年第17期1-5,共5页New Chinese Medicine

基　　金：河北省中医药管理局科研计划项目(2018210);国家级大学生创新创业训练计划项目(201714432001)。

摘　　要：目的:观察车前子对腹泻大鼠小肠组织水通道蛋白3 (AQP3)基因和蛋白表达的影响,探讨其治疗腹泻的作用机制。方法:采用番泻叶灌胃法复制大鼠腹泻模型。将60只SD雄性大鼠随机分为正常组、模型组、氢氯噻嗪组和车前子低、中、高剂量组。除正常组外,其余各组均于每天上午灌胃番泻叶药液,正常组灌胃等量蒸馏水;每天下午各治疗组灌服相应药物,正常组和模型组灌服等量蒸馏水,给药期间观察大鼠的排便情况,连续14 d。实验结束后,根据粪便性状和大便次数,统计对比各组大鼠的稀便率、平均稀便级和腹泻指数。麻醉状态下,剖取2份小肠组织,1份置于冻存管,液氮冻存,用于qRT-PCR法和免疫组化法检测小肠组织AQP3基因和蛋白的表达,1份置于4%多聚甲醛液中固定,苏木精-伊红(HE)染色法观察小肠黏膜病理形态学变化。结果:模型组稀便率、平均稀便级和腹泻指数明显增加(P<0.01);小肠绒毛表皮细胞明显凋亡坏死,固有层毛细血管扩张充血明显,间质有少量中性粒细胞浸润;小肠组织中AQP3基因蛋白的表达水平均下降(P<0.01)。与模型组比较,车前子各剂量组大鼠的稀便率、平均稀便级和腹泻指数均降低(P<0.01);小肠绒毛表皮细胞凋亡、坏死和毛细血管扩张充血现象均减轻,间质有少量中性粒细胞浸润,中、高剂量组可见上皮细胞增生;小肠组织中AQP3基因和蛋白的表达水平均显著高于模型组(P<0.01)。结论:车前子具有止泻作用,其机制与减轻小肠黏膜炎性损伤,上调AQP3基因蛋白的表达水平,增加小肠对水的吸收、调节水液代谢有关。Objective:To observe the effect of semen plantaginis on the expression of AQP3 gene and protein in small intestine tissue of rats with diarrhea,and to explore the mechanism of its treatment of diarrhea. Methods:The rat models with diarrhea were established by intragastric administration of Folium Sennae. Sixty male SD rats were randomly divided into the normal group,the model group,hydrochlorothiazide group and the semen plantaginis groups of low-dose,mediumdose and high-dose. Except for the normal group,all the other groups were given Folium Sennae solution intragastrically every morning,and the normal group was given the same amount of distilled water intragastrically. Each treatment group was given corresponding medicine by gavage every afternoon,while the normal group and the model group were given the same amount of distilled water by gavage. The defecation of rats during the administration period was observed for 14 consecutive days. After the experiment,the loose stool rate,average loose stool grade and diarrhea index of rats in each group were statistically compared according to fecal traits and fecal frequency. Under anesthesia, two small intestine tissues were sected,one of which was placed in cryopreservation tube and cryopreservation with liquid nitrogen for the detection of the expression of AQP3 gene and protein in small intestine by qRT-PCR method and immunohistochemistry;and the other tissue was put in 4% paraformaldehyde solution, and the pathomorphological changes of small intestine mucosa were observed by hematoxylin-eosin(HE) staining. Results:In the model group,the loose stool rate,average loose stool grade and diarrhea index were significantly increased(P<0.01);the villous epidermal cells of small intestine were significantly apoptotic and necrotic;the capillary of lamina propria was dilatation and congestion were obvious,and a few neutrophils were infiltrated in the interstitium;the expression levels of AQP3 gene and protein in small intestine tissue were decreased(P<0.01). Compared with th

关 键 词：车前子 腹泻 水通道蛋白3 基因表达 

分 类 号：R285.5[医药卫生—中药学]