龙葵杂8号向日葵染色体核型分析  

Karyotype Analysis of Chromosome of Sunflower Longkuiza 8

作　　者：马军[1] 丁海燕 辛颖[2] 任国领 黄绪堂 关洪江[1] 王文军[1] 梁春波[1] 徐长君[2] 翟祖欢 MA Jun;DING Haiyan;XIN Ying;REN Guoling;HUANG Xutang;GUAN Hongjiang;WANG Wenjun;LIANG Chunbo;XU Changjun;ZHAI Zuhuan(Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;School of Biological Engineering,Daqing Normal University,Daqing 163712;Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology/Daqing Normal University,Daqing 163712,China)

机构地区：[1]黑龙江省农业科学院经济作物研究所,哈尔滨150086 [2]大庆师范学院生物工程学院,黑龙江大庆163712 [3]黑龙江省油田应用化学与技术重点实验室/大庆师范学院,黑龙江大庆163712

出　　处：《东北农业科学》2021年第4期24-26,78,共4页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省政府博士后资助项目(LBH-Z17204);黑龙江省农业科学院青年基金项目(2018YYYF033);国家特色油料作物产业体系(CARS14-1-06)。

摘　　要：为了探索向日葵染色体核型和染色体制片技术,以龙葵杂8号向日葵根尖为试验材料,探讨低温处理向日葵根尖细胞对染色体制片效果的影响。使用Photoshop和Microsoft Excel软件辅助对龙葵杂8号根尖细胞有丝分裂中期染色体图像进行核型分析。结果表明:低温预处理向日葵根尖细胞容易获得较为理想的可用于核型分析的染色体制片,龙葵杂8号体细胞染色体数目为2n=34,其核型分析公式为:2n=2x=34=24m+6sm+4st。In order to analyze the chromosome karyotype and chromosome production techniques of Longkuiza 8, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using Longkuiza 8 as the experimental material. Photoshop and Microsoft Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of somatic cells of Longkuiza 8 was 2 n=34, whose karyotype analysis formula was 2 n=2 x=34=24 m+6 sm+4 st.

关 键 词：向日葵 染色体 核型分析 龙葵杂8号 

分 类 号：S565.5[农业科学—作物学]