三七总皂苷联合氨基胍治疗对糖尿病肾病大鼠肾功能血CRP TNF-αMDA和SOD水平的影响  

Effects of Panax Notoginseng Saponins Combined with Aminoguanidine Treatment on Renal Function Blood CRP TNF-αMDA and SOD Levels in Diabetic Nephropathy Rats

作　　者：郑杰洪 郑映苗[1] 陈凤梧 ZHENG Jiehong;ZHENG Yingmiao;CHEN Fengwu(The First Affiliated Hospital of Medical College of Shantou University,Guangdong Shantou 515041,China)

机构地区：[1]汕头大学医学院第一附属医院,广东汕头515041

出　　处：《河北医学》2021年第9期1414-1418,共5页Hebei Medicine

基　　金：广东省汕头市科技计划医疗卫生项目,(编号:190531125267683)。

摘　　要：目的:探究三七总皂苷(PNS)联合氨基胍(AG)治疗对糖尿病肾病大鼠肾功能、血C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平的影响。方法:取清洁级雄性SD大鼠75只,随机分为正常对照组(15只)和实验组(60只),实验组腹腔注射链脲佐菌素建立糖尿病肾病大鼠模型,平衡喂养3d,将建模成功的实验组大鼠随机分为模型组(13只)、PNS组(13只)、AG组(13只)、联合组(13只),予以对应药物灌胃4周。观察各组大鼠肾脏组织病理形态变化,并比较各组大鼠肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α、血清MDA、SOD水平。结果:正常对照组肾小球外观规则完整,系膜细胞稀少,基底膜薄;肾小管形状清晰,基底膜无改变,实验组肾小球明显增大,系膜细胞增加,基底膜增厚,肾小管无明显改变;相比模型组,PNS组、AG组、联合组肾小球病理状态明显改善。模型组、PNS组、AG组、联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平高于正常对照组,PNS组、AG组、联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平低于模型组,联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平高于PNS组、AG组:差异均有统计学意义(P<0.05)。模型组、PNS组、AG组、联合组MDA水平高于正常对照组,SOD水平低于正常对照组;PNS组、AG组、联合组MDA水平低于模型组,SOD水平高于模型组;联合组SOD水平高于AG组:差异均有统计学意义(P<0.05)。结论:PNS联合AG治疗对糖尿病肾病可显著改善肾小球病理状态和肾小球功能,降低血浆炎症因子CRP、TNF-α水平,减轻氧化应激水平。Objective:To explore the effects of panax notoginseng saponins combined with aminoguanidine treatment on renal function,blood C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),malondialdehyde(MDA)and superoxide dismutase in diabetic nephropathy rats(SOD)level.Methods:75 clean-grade male SD rats were randomly divided into normal control group(15 rats)and experimental group(60 rats).The experimental group was intraperitoneally injected with streptozotocin to establish a rat model of diabetic nephropathy.The rats in the experimental group were randomly divided into model group(13 rats),PNS group(13 rats),AG group(13 rats),and combination group(13 rats).The rats were given corresponding drugs by gavage for 4 weeks.Observe the pathological changes of rat kidney tissues in each group,and compare the levels of glomerular volume,blood glucose,blood creatinine,24h urine protein,plasma CRP,TNF-α,serum MDA and SOD in each group.Results:The glomerulus in the normal control group had a regular and complete appearance,with few mesangial cells and thin basement membrane;the shape of the renal tubules was clear,and the basement membrane was unchanged.The glomerulus of the experimental group was significantly enlarged,mesangial cells increase,basement membrane thickens,and renal tubules.There was no significant change;compared with the model group,the glomerular pathology of the PNS group,AG group,and combination group was significantly improved.The glomerular volume,blood glucose,blood creatinine,24h urine protein,plasma CRP,and TNF-αlevel of the model group,PNS group,AG group,and combination group were higher than those of the normal control group,PNS group,AG group,and combination group.Blood glucose,blood creatinine,24h urine protein,plasma CRP,and TNF-αlevel were lower than the model group.The combined group had higher glomerular volume,blood glucose,blood creatinine,24h urine protein,plasma CRP,and TNF-αlevel than the PNS group.AG group:The differences were statistically significant(P<0.05).The MDA level of the m

关 键 词：糖尿病肾病 大鼠 肾功能 氧化应激 三七总皂苷 氨基胍 

分 类 号：R285.5[医药卫生—中药学]