白细胞介素32在胆道闭锁患儿肝组织中的表达及与肝纤维化的相关性研究  

Study on the correlation between the expression of interleukin-32 in the liver tissues of children with biliary atresia and liver fibrosis

作　　者：符策君[1] 吴丽珍[2] 陈泽婷 林海[1] 孙为增[1] Fu Cejun;Wu Lizhen;Chen Zeting;Lin Hai;Sun Weizeng(Department of Pediatric Surgery,Hainan General Hospital,Haikou 570311,China;Operating Room,Hainan General Hospital,Haikou 570311,China)

机构地区：[1]海南省人民医院小儿外科,海口570311 [2]海南省人民医院手术室,海口570311

出　　处：《中国医药》2021年第9期1351-1354,共4页China Medicine

基　　金：海南省自然科学基金(20168283);海南省卫生健康行业科研项目(20A200029)。

摘　　要：目的探讨胆道闭锁患儿肝组织中白细胞介素32(IL-32)的表达水平及与肝纤维化的关系。方法选取海南省人民医院2017年2月至2020年6月收治的35例胆道闭锁患儿及14例胆管扩张患儿作为观察组和对照组。比较2组肝组织中IL-32 mRNA和蛋白表达及血清肝纤维化指标水平。分析观察组患儿IL-32表达水平与肝纤维化指标水平的相关性。结果观察组患儿肝组织中IL-32 mRNA和蛋白水平均明显高于对照组[(0.054±0.003)比(0.021±0.002)、(0.58±0.04)比(0.40±0.04)],差异均有统计学意义(均P<0.05)。观察组患儿血清肝纤维化指标Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)、层粘连蛋白(LN)、透明质酸酶(HA)水平均明显高于对照组[(188±23)μg/L比(141±19)μg/L、(161±23)μg/L比(100±12)μg/L、(218±31)μg/L比(143±20)μg/L、(194±25)μg/L比(128±15)μg/L],差异均有统计学意义(均P<0.001)。观察组患儿肝组织中IL-32 mRNA和蛋白表达水平与肝纤维化指标PCⅢ、Ⅳ-C、LN、HA水平均呈正相关(均P<0.05)。结论胆道闭锁患儿肝组织中IL-32表达水平明显升高,且高表达的IL-32与肝纤维化有一定的相关性。Objective To investigate the expression of interleukin-32(IL-32)in liver tissue of children with biliary atresia and its relationship with liver fibrosis.Methods From February 2017 to June 2020,35 children with biliary atresia and 14 children with biliary dilatation admitted to Hainan General Hospital were selected as the observation group and the control group,respectively.The levels of IL-32 mRNA and protein in liver tissue and serum fibrosis indexes levels were compared between the two groups.The correlation between IL-32 level and liver fibrosis indexes level in the observation group was analyzed.Results The levels of IL-32 mRNA and protein in the liver tissue of the observation group were significantly higher than those of the control group[(0.054±0.003)vs(0.021±0.002),(0.58±0.04)vs(0.40±0.04)](both P<0.05).The serum levels of liver fibrosis index procollagenⅢ(PCⅢ),collagenⅣ(Ⅳ-C),laminin(LN)and hyaluronidase(HA)of the observation group were significantly higher than those of the control group[(188±23)μg/L vs(141±19)μg/L,(161±23)μg/L vs(100±12)μg/L,(218±31)μg/L vs(143±20)μg/L,(194±25)μg/L vs(128±15)μg/L](all P<0.001).The expression levels of IL-32 mRNA and protein in liver tissue of observation group were positively correlated with liver fibrosis indexes PCⅢ,Ⅳ-C,LN and HA(all P<0.05).Conclusion The expression level of IL-32 in liver tissue of children with biliary atresia significantly increases,and the high expression of IL-32 is related to liver fibrosis.

关 键 词：胆道闭锁 白细胞介素32 肝纤维化 

分 类 号：R657.44[医药卫生—外科学]