寒温带落叶松天然林不同海拔土壤线虫群落结构的季节动态  

Seasonal Dynamics of Soil Nematode Community Structure in Natural Larch Forests at Different Altitudes in Cold Temperate Zone

作　　者：张岩[1] 杨立学[1] ZHANG Yan;YANG Lixue(School of Forestry,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《森林工程》2021年第5期1-11,共11页Forest Engineering

基　　金：中央高校基本科研业务专项资金(2572019AA07,2572019CP16);黑龙江头雁创新团队计划(森林资源高效培育技术研发团队)。

摘　　要：土壤线虫是土壤微食物网的重要组成部分,为研究沿海拔梯度上土壤线虫群落丰度和多样性的季节动态的变化规律,了解和比较不同时空尺度土壤线虫多样性规律,本文在大兴安岭北部奥克里堆山落叶松天然林中设置6个海拔梯度(750、830、950、1100、1300、1420 m),利用Illumina MiSeq测序方法,探究0~10 cm土壤线虫的群落组成及其结构特征。结果表明,高纬度寒温带地区土壤线虫群落多样性在不同季节均沿海拔梯度呈单调递减的变化,共测定出了1门、3纲、11目、53属,其中食细菌线虫为各个季节和海拔的优势营养类群;海拔梯度对土壤线虫群落结构的影响强于季节变化,随着海拔的增加,Sobs指数(样本总量的指数)和Faith’s PD指数(谱系多样性的指数)均呈降低趋势;土壤pH、可溶性有机碳和氮是影响不同季节土壤线虫海拔分布规律的显著因子。Soil nematode is an important part of the soil micro-food web.In order to study the seasonal dynamics of soil nematode community abundance and diversity along the altitude gradient,and to understand and compare soil nematode diversity at different temporal and spatial scales,in this study,six altitudes(750,830,950,1100,1300,and 1420 m)were set in natural larch forests in the Oakley Mountain in the north of the Greater Khingan Mountains in China,and Illumina MiSeq sequencing method was used to explore the community composition and structural characteristics of soil nematodes in 0-10 cm soil.The results showed that the soil nematode community diversity in the cold temperate zone of high latitude showed a monotonically decreasing pattern along the altitude gradient in different seasons.One phylum,three classes,11 orders,and 53 genera were identified and bacterivores were the dominant trophic groups at each altitude and season.The effect of altitude gradient on soil nematode community structure was stronger than that of seasonal variation.Sobs and Faith's phylogenetic diversity showed a downward trend with the increase in altitude.Soil pH,soluble organic carbon,and soluble organic nitrogen were significant factors affecting the altitude distribution of soil nematodes in different seasons.

关 键 词：土壤线虫 物种丰富度 群落多样性 高通量测序 海拔梯度 季节动态 寒温带 

分 类 号：S791.222[农业科学—林木遗传育种]