改进移动窗口目标转化因子分析算法用于有机磷农药的高通量定性分析  

High-throughput Qualitative Analysis of Organophosphorus Pesticides Based on Improved Moving Window Target Transformation Factor Analysis Algorithm

作　　者：黎才婷 杨清华 雷紫依 赵芷岚 李脉泉 刘霞[1] 杜国荣[4] 李跑 LI Cai-ting;YANG Qing-hua;LEI Zi-yi;ZHAO Zhi-lan;LI Mai-quan;LIU Xia;DU Guo-rong;LI Pao(College of Food Science and Technology,Hunan Provincial Key Laboratory of Food Science and Biotechnology,Hunan Agricultural University,Changsha 410128,China;China Certification&Inspection Group Hunan Co.,Ltd,Changsha 410021,China;Orient Science&Technology College of Hunan Agricultural University,Changsha 410128,China;Beijing Work Station,Technology Center,Shanghai Tobacco Group Co.Ltd,Beijing 101121,China;Hunan Agricultural Product Processing Institute,Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室,湖南长沙410128 [2]中国检验认证集团湖南有限公司,湖南长沙410021 [3]湖南农业大学东方科技学院,湖南长沙410128 [4]上海烟草集团有限责任公司技术中心北京工作站,北京101121 [5]湖南省农业科学院湖南省农产品加工研究所,果蔬贮藏加工与质量安全湖南省重点实验室,湖南长沙410125

出　　处：《中国果菜》2021年第9期65-69,共5页China Fruit & Vegetable

基　　金：国家自然科学基金(31601551、32001680);湖南省自然科学基金(2019JJ50240);中国博士后科学基金面上项目(2019M650187);2021年度湖南农业大学东方科技学院院级“大学生创新训练计划项目”(Y12653202114);湖南省教育厅科学研究项目(19C0933)。

摘　　要：目前,气相色谱质谱联用(GC-MS)技术已广泛应用于农药残留检测,但由于农药残留种类繁多,多农药残留GC-MS信号易受到基线漂移、谱峰重叠等干扰的影响,增大了多农药残留的检测难度。本文结合常见农药残留标准质谱库,利用改进移动窗口目标转换因子分析算法,对有机磷农药混合标准样品GC-MS数据进行了高通量解析。结果表明:利用所建立的方法,在10 min的洗脱时间内可以实现17种有机磷农药的准确定性分析,表明该算法结合标准质谱库可以实现多农药残留的高通量检测,为复杂样品多农药残留自动化分析提供了新的途径。Gas chromatography-mass spectrometry(GC-MS)has been widely used in the field of pesticide residue detection.However,there are varieties of pesticide residues,and the signals of multi-pesticide residues are easily disturbed by baseline drift and peak overlap.It increases the difficulty of multi-pesticide residue detection.In this paper,combined with the established standard mass spectrometry library of pesticide residues,the improved moving window target transformation factor analysis algorithm was used to analyze the GC-MS data of mixed standard sample of organophosphorus pesticides.The results showed that the established method could achieve accurate qualitative analysis of 17 organophosphorus pesticides in 10 min elution time.The improved moving window target transformation factor algorithm combined with standard mass spectrometry library could achieve high-throughput detection of multi-pesticide residues,which provided a new way for automatic analysis of multi-pesticide residues in complex samples.

关 键 词：农药残留 高通量分析 改进移动窗口目标转换因子分析 化学计量学 气相色谱质谱联用技术 

分 类 号：S481.8[农业科学—农药学]