不同大白菜品种对不同地区根肿病菌的抗病性评价  

Evaluation on Resistance of Different Chinese Cabbage Varieties to Clubroot Pathogens Occurred in Different Regions

作　　者：马小超 张慧[1] 张淑江[1] 李菲[1] 章时蕃[1] 李国亮[1] 刘希童 孙日飞[1] MA Xiaochao;ZHANG Hui;ZHANG Shujiang;LI Fei;ZHANG Shifan;LI Guoliang;LIU Xitong;SUN Rifei(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国蔬菜》2021年第9期41-47,共7页China Vegetables

基　　金：国家重点研发计划项目(2017YFD0101802);农业农村部园艺作物生物学与种质创制重点实验室项目,中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)。

摘　　要：为建立快速、稳定的大白菜根肿病苗期抗性鉴定技术,探究大白菜商品种对不同来源根肿病菌的抗性表现,本试验以大白菜根肿病高感品种菊心为试材,以北京顺义根肿病菌为接种菌源,比较优化了注射接种法和浸根接种法的接种条件;采用优化后的浸根接种法,以河南南阳、北京顺义、重庆武隆根肿病菌为菌源,对15个抗根肿病大白菜品种进行人工接种抗性鉴定。结果表明:采用注射接种法,2 mL以上注射量相对于1 mL注射量接种效果显著;采用浸根接种法,根系浸泡5 min以上时,接种效果最佳。不同大白菜品种对三地菌源的抗性表现不一致,其中CR惠民、CR利民和德高CR117对三地根肿病菌均表现较强的抗性,CR高冷地、CR根宝、CR金佰利、德高CR1138、根王、万清克肿皇表现出垂直抗性。In order to establish a rapid and stable technique for identifying Chinese cabbage resistance to clubroot at seedling stage,and explore the resistance performance of commercial Chinese cabbage varieties to clubroot pathogens from different sources,this experiment took the highly susceptible variety of Chinese cabbage‘Juxin’as test material,and clubroot pathogen from Beijing Shunyi as inoculation bacteria source,compared and optimized the inoculation conditions of injection inoculation method and root-dip inoculation method.Adopting the optimized root-dip inoculation method,this experiment also carried out artificial inoculated resistance identification on 15 Chinese cabbage varieties resistant to clubroot with clubroot pathogen from Henan Nanyang,Beijing Shunyi,and Chongqing Wulong as bacteria source.The results showed that when using the injection inoculation method,the injection volume over 2 mL had significant effect in comparison with injection volume of 1 mL,and when using the root-dip inoculation method,soaking root system over 5 min had the best inoculation effect.Different cabbage varieties showed inconsistent resistance performance to bacterial sources from 3 regions,among them‘CR Huimin’‘CR Limin’and‘Degao CR117’all had stronger resistance performance to clubroot pathogens from the 3 regions.While,‘CR Gaolengdi’‘CR Genbao’‘CR Jinbaili’‘Degao CR1138’‘Genwang’‘Wanqingkezhonghuang’showed vertical resistance.

关 键 词：根肿病 大白菜 浸根接种法 注射接种法 抗性鉴定 

分 类 号：S436.341[农业科学—农业昆虫与害虫防治]