亲水相互作用色谱高效分析人尿液中的ATP  

High-efficiency analysis of adenosine triphosphate in human urine by hydrophilic interaction chromatography

作　　者：杨怡 王丹[1] 刘智敏[1] 许志刚[1] YANG Yi;WANG Dan;LIU Zhi-min;XU Zhi-gang(Faculty of Science,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学理学院,云南昆明650500

出　　处：《化学研究与应用》2021年第9期1800-1804,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21565018)资助;云南省教育厅科学研究基金项目(2021Y114)资助;昆明理工大学分析测试基金项目(2019B201811104227、2019B201711104111)资助。

摘　　要：建立了亲水相互作用色谱法高效分析人体尿液中三磷酸腺苷(ATP)的方法。ATP是高能磷酸盐化合物,一种不可替代的生物分子。在本文中,分别使用反相色谱柱和亲水相互作用色谱柱测定ATP。结果表明:在亲水相互作用(HILIC)色谱分离模式下,流动相为甲醇-0.1%的甲酸水溶液=10∶90(v/v)、柱温30℃、流速0.4 mL·min^(-1)、检测波长258 nm时,对ATP的分离效果最佳。在此条件下,测定人尿液中的ATP含量,并在部分样品中的检出1.04~1.65 mg·L^(-1)。进一步加标试验的回收率在77.09%~112.83%之间,相对标准偏差低于13.50%。A method for the efficient analysis of adenosine triphosphate(ATP)in human urine was established by hydrophilic interaction chromatography.ATP is a high-energy phosphate compound,an irreplaceable biological molecule.Herein,reversed-phase chromatography column and hydrophilic interaction chromatography column were used to determine ATP.The results showed that in the HILIC separation mode,the mobile phase was methanol-0.1%formic acid aqueous solution=10∶90(v/v),column temperature 30℃,flow rate 0.4 mL·min^(-1),wavelength 258 nm,the separation effect of ATP was the best.Under these conditions,the ATP content in human urine was determined,and 1.04~1.65 mg·L^(-1) was detected in some samples.The recoveries of the further standard addition test were between 77.09%~112.83%,and the relative standard deviation was lower than 13.5%.

关 键 词：亲水相互作用色谱柱 C18柱 ATP 尿液 

分 类 号：O657.7[理学—分析化学]