细胞治疗产品非临床活体示踪方法研究进展  

Research progress in preclinical in vivo tracer technology for cell therapy products

作　　者：王舒哲 许波华 王雁 黄芳华 邱云良 WANG Shu-zhe;XU Bo-hua;WANG Yan;HUANG Fang-hua;QIU Yun-liang(College of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,China;China Academy of Pharmaceutical Industry Shanghai Innos Biotechnology Co.,Ltd.,Shanghai 201203,China;Innos Biotechnology Nantong Co.Ltd.,Nangtong 226133,China;Center for Drug Evaluation,National Medical Products Administration,Beijing 100022,China)

机构地区：[1]上海工程技术大学化学化工学院,上海201620 [2]中国医药工业研究总院上海益诺思生物技术股份有限公司,上海201203 [3]益诺思生物技术南通有限公司,江苏南通226133 [4]国家药品监督管理局药品审评中心,北京100022

出　　处：《中国药理学与毒理学杂志》2021年第8期624-631,共8页Chinese Journal of Pharmacology and Toxicology

基　　金：国家重点研发计划(2020YFA0112600)。

摘　　要：细胞治疗作为肿瘤学研究和临床实践中的新型疗法,通过修复受损组织或替换身体缺陷细胞,在多种疾病治疗中发挥着越来越重要的作用。但对细胞治疗产品治疗疾病的机制和细胞移植后的宿主体内的分布和迁移情况并不非常清楚。细胞治疗产品的组织分布和存续时间是影响其有效性和安全性的重要因素,需进行动态观察。目前可选择的示踪技术包括影像学、聚合酶链反应(PCR)和免疫组织化学等技术。PCR技术和免疫组织化学技术的缺点是需要在细胞移植后处死动物,取出相应组织进行检测,存在一定的局限性。随着影像学技术的发展,正电子发射计算机断层成像、单光子发射计算机断层成像、磁共振成像和光学成像等手段为细胞移植后无创和实时评估移植细胞的分布、迁移和归巢提供了新选择。近年来,已有大量关于细胞非临床无创性示踪方法的研究。本综述主要对细胞非临床活体示踪方法的研究进展进行综述,从而为细胞治疗产品的组织分布、迁移和归巢研究提供参考。Cell therapy,as a novel therapy in oncology research and clinical practice,plays an increasingly important role in the treatment of a variety of diseases by repairing damaged tissues or replacing defective cells in the body.However,the mechanisms by which cell therapy products treat diseases and their distribution and migration within the host after cell transplantation are not well understood.The tissue distribution and survival time of cell therapy products are important factors affecting their effectiveness and safety of cell therapy products,and dynamic observations should be made.Currently,alternative technical methods include imaging techniques,polymerase chain reaction techniques,and immunohistochemistry,but the disadvantages of polymerase chain reaction techniques and immunohistochemistry lie in the need to sacrifice the animal after cell transplantation and remove the corresponding tissue for testing,which has some limitations.With the development of imaging techniques,positron emission tomography,single photon emission computed tomography,magnetic resonance imaging,and optical imaging have allowed noninvasive and real-time assessment of the distribution,migration,and homing of transplanted cells after cell transplantation.In recent years,there have been numerous studies on cell preclinical noninvasive tracing methods.This review is a summary of the progress made in preclinical intravital tracing methods of cells,thereby providing reference for tissue distribution,migration and homing studies of cell therapies.

关 键 词：细胞治疗 细胞标记 活体示踪 细胞分布 细胞迁移 

分 类 号：R965.2[医药卫生—药理学]