Organoids: a novel modality in disease modeling  

作　　者：Zahra Heydari Farideh Moeinvaziri Tarun Agarwal Paria Pooyan Anastasia Shpichka Tapas KMaiti Peter Timashev Hossein Baharvand Massoud Vosough 

机构地区：[1]Department of Stem Cells and Developmental Biology,Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology,ACECR,Tehran 14155-4364,Iran [2]Department of Developmental Biology,University of Science and Culture,Tehran 14155-4364,Iran [3]Department of Biotechnology,Indian Institute of Technology,Kharagpur,West Bengal 721302,India [4]World-Class Research Center“Digital Biodesign and Personalized Healthcare”,Sechenov First Moscow State Medical University,19991 Moscow,Russia [5]Institute for Regenerative Medicine,Sechenov University,119991 Moscow,Russia [6]Chemistry Department,Lomonosov Moscow State University,119991 Moscow,Russia [7]Department of Polymers and Composites,N.N.Semenov Federal Research Center for Chemical Physics,Russian Academy of Sciences,119991 Moscow,Russia [8]Department of Regenerative Medicine,Cell Science Research Centre,Royan Institute for Stem Cell Biology and Technology,ACECR,Tehran 14155-4364,Iran

出　　处：《Bio-Design and Manufacturing》2021年第4期689-716,共28页生物设计与制造（英文）

基　　金：supported by the National Cancer Control Charity Foundation(Registration Number 41476,Grant Number,235);Iran National Science Foundation,INSF(Grant Number,97014445);by the Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers"Digital biodesign and personalized healthcare"(No.075-15-2020-926).

摘　　要：Limitations of monolayer culture conditions have motivated scientists to explore new models that can recapitulate the architecture and function of human organs more accurately.Recent advances in the improvement of protocols have resulted in establishing three-dimensional(3D)organ-like architectures called‘organoids’that can display the characteristics of their corresponding real organs,including morphological features,functional activities,and personalized responses to specific pathogens.We discuss different organoid-based 3D models herein,which are classified based on their original germinal layer.Studies of organoids simulating the complexity of real tissues could provide novel platforms and opportunities for generating practical knowledge along with preclinical studies,including drug screening,toxicology,and molecular pathophysiology of diseases.This paper also outlines the key challenges,advantages,and prospects of current organoid systems.

关 键 词：Organoid Germ layer Disease modeling Drug screening 

分 类 号：R318[医药卫生—生物医学工程]