杨树菇凝集素诱导C57BL/6小鼠肝损伤和活化CD8+T细胞  

Liver Injury and Activation of CD8+T Cells Induced by Lectin of Agrocybe aegerita in C57BL/6 Mice

作　　者：李岳亮 陈丽琼 文钰棣 黄壮霖 曾淑娴 梁一 LI Yue-liang;CHEN Li-qiong;WEN Yu-di;HUANG Zhuang-lin;ZENG Shu-xian;LIANG Yi(Institute of Laboratory Medicine,Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,School of Medical Technology,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学医学技术学院检验医学研究所,广东省医学分子诊断重点实验室,广东东莞523808

出　　处：《现代食品科技》2021年第9期17-24,共8页Modern Food Science and Technology

基　　金：国家自然科学基金项目(81102850)。

摘　　要：该研究探究了食用蘑菇杨树菇凝集素AAL(Agrocybe aegerita lectin)对小鼠肝损伤的作用机制。通过小鼠尾静脉注射AAL的方式,根据不同的处理时间分0 h、6 h和9 h三个实验组,分别检测其血清AST(天门冬氨酸氨基转移酶)和ALT(丙氨酸氨基转移酶),对肝脏组织切片进行HE染色,RT-PCR检测肝脏细胞因子表达,流式细胞术对肝脏细胞进行分析。与0 h组相比,对小鼠尾静脉注射AAL 6 h和9 h后,小鼠血清ALT和AST显著升高(p<0.001);肝组织切片HE染色发现小鼠肝脏呈现明显的肝损伤,可见坏死区和免疫细胞浸润。注射AAL 6 h后肝脏细胞因子IL-10、IL-33、IL-27、IFN-γ和TNF-α的mRNA表达分别升高到25.14倍、4.62倍、5.14倍、16.51倍和28.10倍。6 h组小鼠肝脏中的T细胞、NKT细胞、CD8+T细胞比例分别由27.92%显著升高到45.95%(p<0.05)、2.46%显著升高到6.36%(p<0.05)、17%显著升高到27.15%(p<0.05)。活化的CD69+T细胞和CD69+CD8+T细胞的比例分别由10.02%显著升高到18.28%(p<0.01)和5.96%显著升高到16.20%(p<0.01)。结果表明食用菌杨树菇AAL可诱导小鼠肝损伤,活化肝脏CD8+T细胞,显著上调细胞因子IL-10、IL-33、IL-27、IFN-γ和TNF-α的表达,提示食用菌凝集素成分是需要考虑安全使用剂量的。The mechanism of the lectin in edible mushroom Agrocybe aegerita(Agrocybe aegerita lectin,AAL)underlying its action on the liver injury in C57BL/6 mice was investigated.C57BL/6 mice were injected by AAL through the tail vein.According to different times of treatment,the mice were divided into three groups:0 h,6 h and 9 h,and the serum ALT(Alanine aminotransferase)and AST(Aspartate aminotransferase)were measured,and the liver tissue sections were stained with HE,and the expression of liver cytokines were detected by RT-PCR,and he liver cells were analyzed by flow cytometry.At 6 h and 9 h groups,the 6 h and 9 h injection of AAL into the tail vein of mice significantly increased the serum ALT and AST(p<0.001);HE staining of liver tissue sections showed obvious liver damage,with necrotic areas and lymphocyte infiltration.Compared with the 0 h group,the serum ALT and AST of mice were significantly increased(p<0.001);the mRNA expression of cytokines IL-10,IL-33,IL-27,IFN-γand TNF-αin the liver of the 6 h mouse group significantly increased by 25.14 times,4.62 times,5.14 times,16.51 times and 28.10 times,respectively.The proportions of T cells,NKT cells,and CD8+T cells in the liver of the 6 h group significantly increased from 27.92%to 45.95%(p<0.05),2.46%to 6.36%(p<0.05),and 17%to 27.15%(p<0.05),respectively.The proportions of activated CD69+T cells and CD69+CD8+T cells in the liver of the 6 h group significantly increased from 10.02%to 18.28%(p<0.01),and 5.96%to 16.20%(p<0.01),respectively.These results showed that AAL induced liver injury in mice,activated CD8+T cells,and significantly upregulated the mRNA expression of the liver cytokines IL-10,IL-33,IL-27,IFN-γand TNF-α,suggesting that there is a need to consider the dose of the fungal lectin for safe use.

关 键 词：杨树菇 凝集素 肝损伤 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]