紫朱软膏对下肢静脉性溃疡成纤维细胞的影响及其机制研究  

Influence and Mechanism Research of Zizhu Ointment on the Fibroblasts of Venous Leg Ulcer

作　　者：杨晓[1] 黄仁燕 李文惠[1] 樊炜静 徐烽 闫少庆[1] 柳国斌[1] YANG Xiao;HUANG Renyan;LI Wenhui;FAN Weijing;XU Feng;YAN Shaoqing;LIU Guobin(Department of Surgery,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院中医血管外科,上海201203

出　　处：《医药导报》2021年第10期1324-1329,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(81804095);国家自然科学基金面上项目(81774310);上海中医健康服务协同创新中心项目(ZYJKFW201701002)。

摘　　要：目的探讨紫朱软膏对TGF-β1/Smads信号通路相关蛋白表达的影响。了解紫朱软膏促进下肢静脉性溃疡(VLUs)愈合的可能机制。方法将42只新西兰雄性大白兔按照随机数字表法分为7组,每组6只:一般溃疡组(d组)、VLUs模型组(c组)、VLUs+基质组(e组)、VLUs+生长因子组(b组)、VLUs+紫朱软膏低浓度组(a1组)、VLUs+紫朱软膏中浓度组(a2组)、VLUs+紫朱软膏高浓度组(a3组),分别在造模成功后第1,7,21天取材,运用苏木精-伊红(HE)染色观察疮面组织病理学改变及蛋白免疫印迹法检测新西兰大白兔下肢疮面组织中转化生长因子-β(TGF-β)、p-Smad2、p-Smad3、α-平滑肌动蛋白(α-SMA)、Ⅰ、Ⅲ型胶原蛋白(CollagenⅠ、CollagenⅢ)、PCNA、Caspase3、Smad7蛋白的表达变化。结果成纤维细胞结构增多数量:a2组>a3组>b组>a1组>e组>d组>c组。a2组与b组p-Smad3、p-Smad2、α-SMA、PCNA、TGF-β1、CollagenⅢ、CollagenⅠ含量最高,d组含量最低,VLUs模型紫朱软膏组与d组比较差异有统计学意义(P<0.05),a2组与c组,e组比较,均差异有统计学意义(均P<0.05)。a2组Smad7、Caspase3蛋白水平最低,d组表达最高,与d组比较,a1,a2,a3组差异有统计学意义(均P<0.05)。结论紫朱软膏对VLUs的修复作用可能与其调节TGF-β1/Smads信号转导通路有关。Objective To study the effects of Zizhu ointment on protein expression based on TGF-β1/Smads signal pathways,and to understand the mechanisms of Zizhu ointment on venous leg ulcer(VLUs).Methods A total of 42 male white rabbits were randomly divided into 7 groups,normal ulcer group(d group),VLUs(lower limb venous ulcer)model group(c group),VLUs+matrix(e group),VLUs+growth factor(b group),VLUs+low concentration of Zizhu ointment(a1 group),VLUs+medium concentration of Zizhu ointment(a2 group),and VLUs+high concentration of Zizhu ointment(a3 group),and 6 rabbits were in each group.Samples were taken on the 1st,7th and 21st days after successful modeling.Histopathology change was determined by hematoxylin eosin(HE)staining,and Western blotting was used to detect the expression changes of transforming growth factor-beta(TGF-beta),p-Smad3,p-Smad3 and alpha smooth muscle protein(alpha SMA),typeⅠandⅢCollagen(CollagenⅠ,CollagenⅢ),PCNA,Caspase3,and Smad7 protein.Results The ranking of the increased number of fibroblasts were as follows,a2 group>a3 group>b group>a1 group>e group>d group>c group.The protein levels of PCNA,TGF-β1,p-Smad2,p-Smad3,Collagen I,Collagen III,and alpha SMA in a2 group and b group were the highest,and those in d group were the lowest.Compared with d group,differences in a1,a2,a3 groups were statistically significant(P<0.05);Compared with c and e group,differences in a2 group were statistically significant(P<0.05).Smad7 and Caspase3 protein levels in a2 group were the lowest,while those in d group were the highest.Compared with d group,differences in a1,a2,and a3 groups were statistically significant(P<0.05).Conclusion The repairing effect of Zizhu ointment on VLUs may be related to its regulation of TGF-β1/Smads signal transduction pathway.

关 键 词：紫朱软膏 下肢静脉性溃疡 成纤维细胞 TGF-β1/Smads信号通路 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]