长江下游麦区新育成品种(系)3种主要病害的抗性鉴定及抗病基因/QTL的分子检测  

Evaluation and molecular detection of three major diseases resistance of new bred wheat varieties(lines)from the lower reaches of the Yangtze River

作　　者：吕国锋[1] 别同德[1] 王慧[1] 赵仁慧[1] 范金平[1] 张伯桥[1] 吴素兰[1] 王玲[1] 汪尊杰[1] 高德荣[1] LYU Guo-Feng;BIE Tong-De;WANG Hui;ZHAO Ren-Hui;FAN Jin-Ping;ZHANG Bo-Qiao;WU Su-Lan;WANG Ling;WANG Zun-Jie;GAO De-Rong(Lixiahe Institute of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement for Lower&Middle Yangtze Valley,Ministry of Agriculture and Rural Affairs,Yangzhou 225007,Jiangsu,China)

机构地区：[1]江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007

出　　处：《作物学报》2021年第12期2335-2347,共13页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0100800);江苏省重点研发计划项目(BE2018350)资助。

摘　　要：小麦赤霉病、白粉病和黄花叶病是长江下游麦区小麦生产的主要病害。本研究对长江下游麦区新育成49个品种(系)的上述3种病害进行抗性鉴定,同时利用与抗赤霉病主效QTL Fhb1和QFhs.crc-2D、抗白粉病基因Pm21以及抗黄花叶病主效QTL QYm.nau-5A.1和QYm.nau-2D连锁的分子标记检测试验品种3种病害抗病基因/QTL组成。结果显示,49.0%的品种赤霉病抗性达中抗以上,32.6%的品种对白粉病免疫或抗,44.9%的品种抗黄花叶病。30.6%和73.6%的试验品种分别含有抗赤霉病主效QTL Fhb1和QFhs.crc-2D,宁麦9号和扬麦158及其衍生品种分别是Fhb1和QFhs.crc-2D的主要载体品种;28.6%的品种含抗白粉病基因Pm21,镇麦9号和扬麦18及其衍生品种为Pm21的主要载体品种;分子检测含抗黄花叶病主效QTL QYm.nau-5A.1和QYm.nau-2D的品种比例均为24.5%,宁麦9号和苏麦6号及其衍生品种分别是QYm.nau-5A.1和QYm.nau-2D的主要载体品种。宁麦9号和扬麦158衍生品种在小麦抗赤霉病和黄花叶病基因/QTL的组成存在分化。结果为长江下游麦区小麦抗病分子育种提供了重要参考。Fusarium head blight(FHB),powdery mildew(PM),and wheat yellow mosaic virus(WYMV)are three major diseases of wheat in the lower reaches of Yangtze River.The resistance to FHB,PM,and WYMV of 49 new bred wheat varieties(ad-vanced lines)from the lower reaches of the Yangtze River were evaluated,and the molecular markers linked to FHB resistance QTL Fhb1 and QFhs.crc-2D,PM gene Pm21 and WYMV resistance QTL QYm.nau-5A.1 and QYm.nau-2D were used to detect the correspond resistance genes/QTLs.49.0%of the varieties were above moderate resistance to FHB,32.6%varieties were im-mune or resistant to PM,and 44.9%varieties were resistant to WYMV.30.6%and 73.6%of the tested varieties contained Fhb1 and QFhs.crc-2D,respectively.Ningmai 9 and Yangmai 158 as well as their derived varieties were the main carriers of Fhb1 and QFhs.crc-2D,respectively.Pm21,a powdery mildew resistant gene,was detected in 28.6%of the tested varieties,Zhenmai 9 and Yangmai 18 were two major donors of Pm21.QYm.nau-5A.1 and QYm.nau-2D,two MYMV resistant QTL,were detected in 24.5%of the tested varieties.Ningmai 9 and Zhenmai 9 were two major QTL donors.The composition of resistance genes/QTLs to FHB and WYMV in Yangmai 158 and their derived varieties diverged from Ningmai 9 and its derived varieties.These results provide the important information for molecular breeding for wheat disease resistance in lower reaches of Yangtze River.

关 键 词：小麦 品种 抗病QTL/基因 分子鉴定 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]