结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因  

Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L.

作　　者：王瑞莉 王刘艳 雷维 吴家怡 史红松 李晨阳 唐章林[1,2] 李加纳 周清元[1,2] 崔翠 WANG Rui-Li;WANG Liu-Yan;LEI Wei;WU Jia-Yi;SHI Hong-Song;LI Chen-Yang;TANG Zhang-Lin;LI Jia-Na;ZHOU Qing-Yuan;CUI Cui(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China;Chongqing Rape Engineering Technology Research Center,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]重庆市油菜工程技术研究中心,重庆400715

出　　处：《作物学报》2021年第12期2407-2422,共16页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2018YFD0100500);国家现代农业产业技术体系建设专项(CARS-12);重庆市技术创新与应用发展项目(cstc2019jscx-msxmX0383)资助。

摘　　要：随着土壤酸化程度的加剧,铝毒害已成为影响种子萌发质量和作物产量的重要胁迫因子之一。为研究铝毒对油菜种子萌发过程影响的分子机理,本文采用RNA-seq技术对耐铝品系18D300和敏铝品系27011进行转录组分析,共检测到9344个显著差异表达基因[|log2(fold change)|≥1和FDR≤0.05],其中4406个DEGs基因上调,4938个DEGs下调。GO富集分析发现,差异表达基因主要与氧化反应、细胞碳水化合物代谢过程、转运蛋白活性等相关。KEGG富集分析表明,差异表达基因主要富集于苯丙烷生物合成、淀粉和蔗糖代谢、MAPK信号通路-植物、植物-病原体相互作用、植物激素信号转导等途径。此外,通过整合RNA转录组测序和铝毒胁迫下油菜萌发期根系相关性状的QTL定位结果,共筛出44个差异表达基因(10个下调和34个上调),这些基因主要与氧化应激、渗透调节、细胞壁修饰、转运蛋白、激素信号传导等功能有关。With the aggravation of soil acidification,aluminum toxicity has become one of the important stress factors affecting seed germination quality and crop yield.In order to study the molecular mechanism of the effect of aluminum toxicity on rapeseed seed germination,a total of 9344 significantly differentially expressed genes[log2(fold change)≥1 and FDR≤0.05]were de-tected in the transcriptome analysis of aluminum-tolerant strain 18D300 and aluminum-sensitive strain 27011 by RNA-seq tech-nology,among which 4406 DEGs(differentially expressed genes)were up-regulated and 4938 DEGs were down-regulated.GO enrichment showed that DEGs were mainly related to oxidation reaction,carbohydrate metabolism,and transporter activity.KEGG enrichment revealed that DEGs were mainly concentrated in phenylpropane biosynthesis,starch and sucrose metabolism,MAPK signal pathway-plant,plant-pathogen interaction,plant hormone signal transduction and so on.In addition,44 DEGs(10 down-regulated and 34 up-regulated)were screened by integrating the results of RNA transcriptome sequencing and QTL map-ping of root-related traits at germination stage under aluminum toxicity stress in rapeseed,which were mainly related to oxidative stress,osmotic regulation,cell wall modification,transporter,and hormone signal transduction.

关 键 词：甘蓝型油菜 铝毒胁迫 转录组测序 QTL 差异表达基因 

分 类 号：S565.4[农业科学—作物学]