黄芪总苷对颅脑损伤大鼠神经细胞氧化应激和凋亡的影响  

Effect of astragaloside on oxidative stress and apoptosis of nerve cells in rats with brain injury

作　　者：杨林[1] 马宁 陈培莉[1] Yang Lin;Ma Ning;Chen Peili(Shangqiu First People′s Hospital,Department of Critical Care Medicine of Xuzhou Medical University Shangqiu Clinical College,Shangqiu 476100,China;Department of Critical Care Medicine,Shanxi Bethune Hospital,Taiyuan 030032,China)

机构地区：[1]商丘市第一人民医院,徐州医科大学商丘临床学院重症医学科,476100 [2]山西白求恩医院重症医学科,太原030032

出　　处：《中华实验外科杂志》2021年第9期1674-1677,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察黄芪总苷对颅脑损伤大鼠神经细胞氧化应激和凋亡的影响。方法 45只SD大鼠按照随机数字表法分为假手术组、模型组和黄芪总苷组,每组15只。采用Feeney’s自由落体法建立颅脑损伤模型。黄芪总苷组大鼠建模后腹腔注射黄芪总苷50 mg/kg。假手术组和模型组腹腔注射等体积生理盐水。两组治疗7 d后,评价3组大鼠神经功能缺失评分;采用试剂盒检测氧化应激指标变化;采用原位缺口末端标记法(TUNEL)染色分析3组大鼠脑组织神经元凋亡比例;采用蛋白质印迹法(Western blot)分析3组大鼠凋亡相关蛋白表达水平。计量资料比较采用方差分析。结果黄芪总苷组大鼠神经功能评分缺失评分[(13.27±3.67)分]明显高于模型组[(6.18±2.87)分],差异有统计学意义(t=3.071,P<0.05)。黄芪总苷组大鼠逃避潜伏期[(31.44±5.71) s]明显低于模型组[(49.27±7.48) s],差异有统计学意义(t=4.019,P<0.05)。黄芪总苷组大鼠穿台次数[(49.33±7.09)次]明显高于模型组[(31.49±5.91)次],差异有统计学意义(t=4.004,P<0.05)。黄芪总苷组大鼠脑组织丙二醛(MDA)水平[(0.57±0.11) nmol/mg]明显低于模型组[(0.98±0.12) nmol/mg],差异有统计学意义(t=2.974,P<0.05)。黄芪总苷组大鼠脑组织超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平[(23.51±3.11)、(8.37±1.23) U/mg]明显低于模型组[(37.32±4.01)、(15.33±2.99) U/mg],差异有统计学意义(t=4.028、3.112,P<0.05)。黄芪总苷组大鼠脑组织神经元凋亡比例[(12.57±3.81)%]明显低于模型组[(20.44±4.10)%],差异有统计学意义(t=5.291,P<0.05)。黄芪总苷组大鼠脑组织半胱氨酰天冬氨酸特异性蛋白酶-3(Caspase-3)相对表达水平和B细胞淋巴瘤/白血病-2相关X蛋白(bax)/B细胞淋巴瘤/白血病-2(bcl-2)比值(1.57±0.13、0.98±0.11)明显低于模型组(2.10±0.14、1.32±0.12),差异有统计学意义(t=2.716、2.291,P<0.05)。结论黄芪总苷可显著下调颅脑损伤大�Objective To investigate the effect of astragaloside on oxidative stress and apoptosis of nerve cells in rats with brain injury.Methods Totally,45 SD rats were divided into sham operation group,model group and astragaloside group according to the random digital table method.The brain injury model was established by free fall method.The rats in the astragaloside group were injected with 50 mg/kg astragaloside intraperitoneally.The sham operation group and model group were injected with equal volume saline.After 7 days of treatment,the scores of neurologic deficit were evaluated in the three groups.The change of oxidative stress index was detected by the kit.The apoptosis rate of neurons in the brain tissue of the three groups was analyzed by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)staining.The expression level of apoptosis related protein in three groups was analyzed by Western blotting.Variance analysis was used to compare the measurement data.Results The neurological deficit score in astragaloside group(13.27±3.67)was significantly higher than that in the model group(6.18±2.87,t=3.071,P<0.05).The escape latency in astragaloside group[(31.44±5.71)s]was significantly shorter than that in the model group[(49.27±7.48)s,t=4.019,P<0.05].The number of rats crossing the platform in astragaloside group[(49.33±7.09)times]was significantly greater than that in the model group[(31.49±5.91)times,t=4.004,P<0.05].The content of malondialdehyde(MDA)in brain tissue in astragaloside group[(0.57±0.11)nmol/mg]was significantly lower than that in the model group[(0.98±0.12)nmol/mg,t=2.974,P<0.05].The activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in brain tissue in astragaloside group[(23.51±3.11),(8.37±1.23)U/mg]was significantly lower than that in model group[(37.32±4.01),(15.33±2.99)U/mg,t=4.028,3.112,P<0.05].The proportion of neuronal apoptosis in brain tissue of astragaloside group[(12.57±3.81)%]was significantly lower than that in model group[(20.44±4.10)%,t=

关 键 词：黄芪总苷 颅脑损伤 神经功能 氧化应激 凋亡 

分 类 号：R285.5[医药卫生—中药学]