不同配比柴胡人参药对对非酒精性脂肪性肝病大鼠糖脂代谢的影响  

Effects of drug pair of Bupleuri Radix and Ginseng Radix et Rhizoma with different proportions on glucose and lipid metabolism in rats with non-alcoholic fatty liver disease

作　　者：张强[1] 刘凯利 金俊[2] 张力[1] 阮君 尚浩南 陈珺明 肖铁刚 王兵[1] ZHANG Qiang;LIU Kaili;JIN Jun;ZHANG Li;RUAN Jun;SHANG Haonan;CHEN Junming;XIAO Tiegang;WANG Bing(Department of Traditional Chinese Medicine,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 200233,China;Department of Gerontology,Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 200233,China;Department of Traditional Chinese Medicine,Zhoupu Hospital Affiliated to Shanghai University of Medicine&Health Sciences,Shanghai 201318y China;Department of Gastroenterology,Shanghai Traditional Chinese Medicine-Inter grated Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)

机构地区：[1]上海交通大学附属第六人民医院中医科,上海200233 [2]上海交通大学附属第六人民医院老年病科,上海200233 [3]上海健康医学院附属周浦医院中医科,上海201318 [4]上海中医药大学附属上海市中西医结合医院消化科,上海200082

出　　处：《上海中医药杂志》2021年第8期80-87,共8页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81774061);国家自然科学基金青年科学基金项目(81704006);上海市科委中医引导项目(16401934200);上海市卫计委中医药科研基金资助项目(2016LP023);上海市中医药传承和科技创新项目(ZYKC2019035);上海市综合医院中西医结合专项(ZHYY-ZXYJHZX-201910);上海市虹口区国医强优三年行动计划项目(HGY-KY-2018-14);上海交通大学教育教学研究项目(JYJX200021);上海交通大学医学院本科教学质量项目(沪交医教[2020]10号);上海市第六人民医院院级医学教育研究课题重点项目。

摘　　要：目的探索不同配伍比例柴胡人参药对对非酒精性脂肪性肝病(NAFLD)大鼠糖脂代谢的影响。方法将48只Wistar大鼠随机分为正常组(C组)8只和造模组40只。C组给予普通饲料及0.9%NaCl溶液灌胃,造模组给予高脂高糖饲料及0.9%NaCl溶液灌胃,造模成功后将造模组大鼠随机分成模型组(M组)、柴胡人参1∶1组(CR 1∶1组)、柴胡人参1∶2.5组(CR 1∶2.5组)、柴胡人参2.5∶1组(CR 2.5∶1组)、奥贝胆酸组(OB组),每组8只。各用药组给予高脂高糖饲料及相应药物灌胃。各组干预结束后称量大鼠体质量、肝湿重,观察病理染色,检测肝功能、血脂、空腹血糖(FBG)、胰岛素敏感指数(ISI)和胰岛素抵抗指数(HOMA-IR)。结果与正常组比较,M组大鼠丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TC)、三酰甘油(TG)、FBG、胰岛素含量(FINS)、胰岛素抵抗指数(HOMA-IR)水平升高,胰岛素敏感指数(ISI)水平下降,肝脏脂肪变性及炎症程度加重。与M组比较,各用药组的糖代谢、脂代谢有一定程度的改善。具体而言,各用药组之间比较,CR 2.5∶1组的体质量、肝湿重,以及TC、ALT、AST、FBG、FINS、HOMA-IR、脂肪变性及炎症评分下降较其余两组显著,ISI水平升高也最显著,故柴胡人参配伍2.5∶1时的药效作用最佳;与OB组比较,CR 2.5∶1组体质量水平下降更为显著,而肝湿重、TC、AST、FBG、ISI及炎症评分的改善不如OB组显著。结论柴胡人参药对可以改善NAFLD大鼠的糖脂代谢,其最佳配伍比例为柴胡与人参2.5∶1。Objective To explore the effects of different compatibility ratios of drug pair of Bupleuri Radix and Ginseng Radix et Rhizoma on glucose and lipid metabolism in non-alcoholic fatty liver disease(NAFLD)rats.Methods Forty-eight Wistar rats were randomly divided into a normal group(group C)with 8 rats and a modeling group with 40 rats.The rats in Group C was given normal feed and 0.9%NaCl solution by gavage,and the rats in the modeling group was given high-fat and high-sugar feed and 0.9%NaCl solution by gavage.After the model was successfully built,the model rats were randomly divided into model group(group M),drug pair of Bupleuri Radix and Ginseng Radix et Rhizoma 1∶1 group(CR 1∶1 group),drug pair of Bupleuri Radix and Ginseng Radix et Rhizoma 1∶2.5 group(CR 1∶2.5 group),drug pair of Bupleuri Radix and Ginseng Radix et Rhizoma 2.5∶1 group(CR 2.5∶1 group)and obeticholic acid group(OB group),8 rats in each group.Each medication group was given high-fat and high-sugar feed and corresponding drugs by gavage.After the intervention,the body weight and liver wet weight of the rats were weighed,the pathological staining was observed,and the liver function,blood lipid,fasting blood glucose(FBG),insulin sensitivity index(ISI)and insulin resistance index(HOMA-IR)were measured.Results Compared with the group C,alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triacylglycerol(TG),FBG,insulin content(FINS)and HOMA-IR increased,while insulin sensitivity index(ISI)level decreased,and liver steatosis and inflammation aggravated in group M of rats.Compared with group M,the glucose metabolism and lipid metabolism of each medication group were improved to a certain extent.Comparison between medication groups,the body weight,liver wet weight,TC,ALT,AST,FBG,FINS,HOMA-IR,and steatosis and inflammation scores of the CR 2.5∶1 group decreased significantly compared with the other two groups;the increase in ISI level is also the most significant;the drug effect is the best when the proportio

关 键 词：柴胡 人参 模型大鼠 非酒精性脂肪性肝病 糖代谢 脂代谢 中药研究 

分 类 号：R285.5[医药卫生—中药学]