当归芍药散对高脂饮食诱导的非酒精性脂肪肝小鼠模型的影响  

Effect of Danggui Shaoyao Powder on mice model with non-alcoholic fatty liver induced by high-fat diet

作　　者：施凯舜 徐峥 岳跃兵 黄洁 SHI Kaishun;XU Zheng;YUE Yuebing;HUANG Jie(Department of Geriatrics,The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310005,China)

机构地区：[1]浙江中医药大学附属第二医院老年科,浙江杭州310005

出　　处：《上海中医药杂志》2021年第9期81-85,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2021ZA069)。

摘　　要：目的观察当归芍药散对非酒精性脂肪肝小鼠模型肝功能、肝脏和血清三酰甘油(TG)水平以及肝组织形态学的影响。方法雄性小鼠40只,随机分为正常组10只及高脂高糖造模组30只。造模成功后将高脂高糖组小鼠随机分为模型组、当归芍药散组和奥贝胆酸对照组。当归芍药散组(25.8 g/kg)和奥贝胆酸对照组(0.6 mg/kg)均灌胃给予相应的药物,正常组与模型组小鼠以等量饮用水灌胃,各组均于6周后取材。测定各组小鼠的体质量、肝湿重、肝脏和血清三酰甘油水平,血清空腹胰岛素(FINS)含量、空腹血糖(FBG)含量、胰岛素抵抗指数(HOMA-IR),血清ALT、AST水平,肝组织病理及肝组织NAS评分。结果与模型组比较,当归芍药散组小鼠体质量、肝湿重显著降低(P<0.01),肝脏和血清TG水平显著降低(P<0.01),血清ALT、AST活性均大幅下降(P<0.01),血清FBG、FINS和HOMA-IR均显著降低(P<0.01),肝组织NAS总分显著下降(P<0.01)。结论当归芍药散可有效抑制小鼠肝脏脂肪沉积,减轻小鼠肝脏炎症损伤。Objective To observe the effect of Danggui Shaoyao Powder on liver function,liver and serum triglyceride(TG)and liver histomorphology of non-alcoholic fatty liver mice.Methods Forty male mice were randomly divided into normal diet group(n=10)and hyperlipidemia-hyperglycemia group(n=30).After the successful establishment of the model,the mice in the hyperlipidemia-hyperglycemia group were randomly divided into model group,Danggui Shaoyao Powder group and obeticholic acid group.The mice in the Danggui Shaoyao Powder group(25.8 g/kg)and the obeticholic acid group(0.6 mg/kg)were given the corresponding drugs by intragastric administration.The mice in the normal group and the model group were fed with the same amount of drinking water.The samples were taken after 6 weeks.The body weight,liver wet weight,liver and serum triacylglycerol levels,fasting insulin(FINS)content,fasting blood glucose(FBG)content,insulin resistance index(HOMA-IR),serum ALT and AST levels,liver histopathology and liver tissue NAS score were measured.Results Compared with the model group,the body weight and wet weight of the liver,the levels of TG in the liver and serum,the activities of ALT and AST in serum,FBG,FINS and HOMA-IR in serum,and the total score of NAS in the liver tissue in Danggui Shaoyao Powder group were significantly lower than those in model group(P<0.01).Conclusion Danggui Shaoyao Powder can effectively inhibit liver fat deposition and reduce liver inflammatory injury in mice.

关 键 词：当归芍药散 非酒精性脂肪肝 模型小鼠 血脂 中药研究 

分 类 号：R285.5[医药卫生—中药学]