中国首例人感染猕猴α疱疹病毒1型病例的确诊  

Confirmation of the first human case with Macacine alphaherpesvirus 1 infection in China

作　　者：赵莉[1] 齐文杰[2] 杜海军[1] 郑阳[3] 刘景院 王文玲[1] 王国兴[5] 潘阳[3] 黄保英[1] 冯兆民 张代涛[3] 杨鹏[3] 陈艳伟[3] 李爽[3] 马春娜[3] 韩俊[1] 王全意[3] 谭文杰[1] Zhao Li;Qi Wenjie;Du Haijun;Zheng Yang;Liu Jingyuan;Wang Wenling;Wang Guoxing;Pan Yang;Huang Baoying;Feng Zhaomin;Zhang Daitao;Yang Peng;Chen Yanwei;Li Shuang;Ma Chunna;Han Jun;Wang Quanyi;Tan Wenjie(Key Laboratory of Biosafety of National Health Commission,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China;Department of Infectious Diseases,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Beijing Center for Disease Prevention and Control,Beijing 100013,China;Department of Critical Care Medicine,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China;Emergency Department,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委员会生物安全重点实验室,北京102206 [2]首都医科大学附属北京友谊医院感染内科,100050 [3]北京市疾病预防控制中心,北京市预防医学研究中心传染病地方病控制所,100013 [4]首都医科大学附属北京地坛医院重症医学科,100015 [5]首都医科大学附属北京友谊医院急诊科,100050

出　　处：《国际病毒学杂志》2021年第4期278-282,共5页International Journal of Virology

基　　金：国家重点研发计划(2016YFD0500301)。

摘　　要：目的对发生在中国的首例疑似猕猴α疱疹病毒1型感染者进行实验室确诊。方法采集疑似感染者的脑脊液进行高通量测序,并采集疑似感染者的脑脊液、痘疱液、鼻咽拭子、结痂涂抹、唾液等标本以及密切接触者的痘疱液、口咽拭子、血清等标本后,利用4套实时定量PCR方法检测猕猴α疱疹病毒1型、水痘-带状疱疹病毒、猴痘病毒和正痘病毒。结果疑似感染者的脑脊液高通量测序结果检出285个猕猴α疱疹病毒样序列读数;猕猴α疱疹病毒1型特异性实时定量PCR检测患者脑脊液、结痂涂抹和唾液标本为阳性,疑似感染者的其余样本及密切接触者的样本均为阴性。水痘带状疱疹、猴痘和正痘病毒荧光定量PCR检测所有样本均为阴性。结论患者脑脊液、结痂涂抹和唾液标本猕猴α疱疹病毒1型核酸检测为阳性,结合病人的临床特征、动物接触史及高通量测序结果,确诊该患者为猕猴α疱疹病毒1型感染。Objective To diagnose the first suspected case of macacine alphaherpesvirus 1(herpes B-virus,BV)infection in Beijing,China.Methods Specimens of cerebrospinal fluid(CSF)of a suspected infected person was collected for next-generation sequencing(NGS),and the specimens,including the CSF,blister fluid,nasopharyngeal swab,scab swab and saliva were collected from the suspected infected case.The specimens,including blister fluid,throat swab and serum were collected from two close contacts.Four sets of quantitative real-time PCR(qPCR)were performed to detect macacine alphaherpesvirus 1,varicella zoster virus(VZV),monkeypox virus(MPXV)and orthopoxvirus(OPV).Results A total of 285 alpha-herpesvirus-like sequence reads were found in CSF of suspected case by NGS.The samples of CSF,scab swab and saliva of the patient were positive for macacine alphaherpesvirus 1 by qPCR while the other specimens from the patient and all the specimens from the two close contacts were all negative for macacine alphaherpesvirus 1.Moreover,all tests were negative for VZV,MPXV and OPV.Conclusions The tests for macacine alphaherpesvirus 1 in CSF,scab swab and saliva samples from the patient were positive.The first human infection case with macacine alphaherpesvirus 1 was confirmed by NGS and qPCR,together with the clinical characteristics,animal contact history.

关 键 词：中国 猕猴α疱疹病毒1型 实时定量PCR 疱疹病毒 首例 

分 类 号：R511[医药卫生—内科学]