肿瘤坏死因子超家族15-358T>C变异与食管癌发病风险的相关性  

Association of tumor necrosis factor superfamily 15-358T>C variant and risk of esophageal cancer

作　　者：车玲玉 李昂 谢俞宁 高慧 仵红娇 贾祯贤 李佳莹 张雪梅 CHE Ling-yu;LI Ang;XIE Yu-ning;GAO Hui;WU Hong-jiao;JIA Zhen-xian;LI Jia-ying;ZHANG Xue-mei(College of Life Science,North China University of Science and Technology,Tangshan 063210,Hebei Province,China;School of Public Health,North China University of Science and Technology,Tangshan 063210,Hebei Province,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210 [2]华北理工大学公共卫生学院,河北唐山063210

出　　处：《中国临床药理学杂志》2021年第17期2262-2265,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究肿瘤坏死因子超家族15(TNFSF15)遗传变异对食管癌发病风险的影响。方法利用UALCAN数据库分析食管癌组织中TNFSF15的表达及临床病理分期。选取700例食管癌患者为病例组、700例健康体检者为对照组,用聚合酶链反应限制性片段长度多态性技术对TNFSF15启动子区-358T>C(rs647810^(9))及-638A>G(rs7848647)遗传变异进行基因分型。用非条件Logistic回归计算比值比(OR值)及95%可信区间(95%CI)分析TNFSF15启动子区-358T>C及-638A>G遗传变异各基因型与食管癌发病风险之间的关系。结果 TNFSF15在食管癌组织中高表达(P<0.001),其高表达与食管癌临床病理分期的早期显著相关(P<0.01),但对食管癌转移无显著影响(P>0.05)。病例组和对照组中TNFSF15-358CC基因型携带者分别为304例(43.4%)和232例(33.2%),是TT基因型携带者食管癌发病风险的1.39倍(OR=1.39,95%CI=1.03~1.88,P<0.05),而病例组和对照组中TNFSF15-358TC基因型携带者分别为262例(37.5%)和320例(45.7%),与TT基因型携带者相比对食管癌发病风险无显著影响(OR=0.87,95%CI=0.64~1.17,P>0.05)。分层分析显示,与高年龄组TNFSF15-358TT基因型携带者相比,CC基因型携带者食管癌发病风险增加1.60倍(OR=1.60,95%CI=1.02~2.51,P<0.05)。与非吸烟者中TNFSF15-358TT基因型携带者相比,CC基因型携带者患食管癌的风险增加1.78倍(OR=1.78,95%CI=1.16~2.72,P<0.05)。与不同性别TNFSF15-358TT基因型携带者相比,CC基因型携带者对食管癌发病风险无明显影响(P>0.05)。TNFSF15-638A>G遗传变异与食管癌的发病风险无关(P>0.05)。结论 TNFSF15-358T>C遗传变异是食管癌的遗传易感因素。Objective To investigate the effect of genetic variation of tumor necrosis factor superfamily 15(TNFSF15) on the risk of esophageal cancer. Methods The relationship of the TNFSF15 expression and clinicopathological stages of esophageal cancer tissues were analyzed by UALCAN database. The case-control study involved in 700 patients with esophageal cancer and 700 health controls. The TNFSF15-358 T>C(rs647810^(9)) and-638 A>G(rs7848647) polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism. The odds ratio(OR) and 95% confidence interval(95% CI) were calculated by unconditional logistic regression. The relationship between TNFSF15-358 T>C or-638 A>G genetic variation and the risk of esophageal cancer was evaluated. Results TNFSF15 was highly expressed in esophageal cancer tissues( P < 0. 001), and its high expression was significantly correlated with the early stage of esophageal cancer( P < 0. 01),but there was no significant effect on esophageal cancer metastasis( P > 0. 05). There were 304 cases( 43. 4%) and 232 cases( 33. 2%) carriers of TNFSF15-358 CC genotype in case group and control group,compared with-358 TT genotype carriers,-358 CC genotype carriers had an 1. 39 times increased risk of esophageal cancer( OR = 1. 39,95% CI = 1. 03-1. 88,P < 0. 05). The TNFSF15-358 TC genotype carriers were 262 cases( 37. 5%) and 320 cases( 45. 7%) in the case group and the control group,there was no significant effect on the risk of esophageal cancer compared with TT genotype carriers( OR = 0. 87,95% CI = 0. 64-1. 17,P > 0. 05). The age stratified analysis showed that the elderly people risk of esophageal cancer was increased 1. 60 times among elders with TNFSF15-358 CC genotype compared with those with-358 TT genotype( OR = 1. 60,95% CI = 1. 02-2. 51,P < 0. 05). Smoking stratified analysis exhibited that the risk of esophageal cancer was increased 1. 78 times among non-smokers with TNFSF15-358 CC genotype compared with those with-358 TT genotype( OR = 1. 78,95% CI =1. 16-2. 72,P

关 键 词：肿瘤坏死因子超家族15 遗传变异 食管癌 易感性 单核苷酸多态性 

分 类 号：R97[医药卫生—药品]