镰形棘豆总黄酮的含量测定方法研究  

Study on the determination of total flavonoids in Oxytropis falcata Bunge

作　　者：刘晓玲[1] 杨育儒 蔡小辉 陈锦珊[1] LIU Xiaoling;YANG Yuru;CAI Xiaohui;CHEN Jinshan(Department of Pharmacy,No.909 Hospital of Joint Logistics Support Force of the PLA/Southeast Hospital Affiliated to Xiamen University,Zhangzhou 363000,China)

机构地区：[1]中国人民解放军联勤保障部队第909医院/厦门大学附属东南医院药剂科,福建漳州363000

出　　处：《药学实践杂志》2021年第5期422-425,459,共5页Journal of Pharmaceutical Practice

基　　金：原南京军区科技创新项目(14MS085);漳州市自然科学基金(ZZ2018J15);第九〇九医院青年苗圃项目(NO.17Y012)。

摘　　要：目的建立紫外-可见分光光度法测定镰形棘豆总黄酮含量的方法。方法分别采用直接测定法、氯化铝-醋酸钠显色法和硝酸铝显色法,以芦丁为对照品,探究最优显色方法并进行显色条件优化和系统的方法学考察。结果最优显色法为氯化铝-醋酸钠显色法,以供试品不加显色剂为参比,0.3 mol/L三氯化铝溶液3.0 ml,1.0 mol/L醋酸钠4.0 ml,放置16 min,最大吸收波长为277 nm,此方法项下芦丁对照品在8.8~44μg/ml的范围内,浓度与吸光度呈现良好的线性关系,线性回归方程为Y=0.0353X+0.0267(r=0.9996),加样回收率平均值为100.91%(n=9,RSD=2.426%)。结论该方法简便、快速、准确、灵敏,可作为镰形棘豆总黄酮含量的测定方法。Objective To establish an UV spectrophotometry method for the determination of total flavonoids in Oxytropis falcata Bunge.Methods Using rutin as comparison,three coloration methods were used to find the optimal assay method,optimize the color conditions and take a systematic methodological investigation.Results The best color development method is aluminum chloride-sodium acetate color method.Using the test product without adding color reagent as a reference,0.3 mol/L aluminum chloride solution 3.0 ml,1.0 mol/L sodium acetate 4.0 ml,placed for 16 min,the sample was detected at 277 nm wavelength by AlCl3-CH3COONa reaction by using rutin as reference.The rutin content had a good liner relationship in the range of 8.8 to 44μg/ml(r=0.9996),and the average recovery rate was 100.91%with RSD of 2.426%.Conclusion This method is simple,rapid,accurate and sensitive and can be used as a method for the determination of total flavonoids from Oxytropis falcata Bunge.

关 键 词：镰形棘豆 总黄酮 含量测定 紫外-可见分光光度法 氯化铝-醋酸钠显色法 

分 类 号：R917[医药卫生—药物分析学]