用HPLC法测定红茴香注射液中槲皮苷的含量  

Quantitative determination of quercetin in Honghuixiang injection by HPLC

作　　者：成程 陈向梅 马健[1] 赵氚 CHENG Cheng;CHEN Xiangmei;MA Jian;ZHAO Chuan(Department of Pharmacy,Medical Security Centre,Chinese PLA General Hospital,Beijing 100048,China)

机构地区：[1]中国人民解放军总医院医疗保障中心药剂科,北京100048

出　　处：《药学实践杂志》2021年第5期447-448,453,共3页Journal of Pharmaceutical Practice

摘　　要：目的建立高效液相色谱法测定红茴香注射液中槲皮苷的含量。方法色谱柱为Dikma C18(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸水溶液(25∶75)为流动相,流速为1.0 ml/min,柱温30℃,检测波长为256 nm。结果槲皮苷在0.215~3.225μg范围内呈良好线性关系,相关系数为0.999 6,该法的平均回收率为99.39%,RSD为0.82%(n=6),重复性为1.194 mg/ml,RSD为0.40%。结论 3批红茴香注射液的槲皮苷平均含量结果为1.191 mg/ml,该法简便、快速、准确,可作为测定红茴香注射液中槲皮苷含量的方法。Objective To establish quantitative methods to assay quercetin in Honghuixiang injection by HPLC.Methods Dikma C18 column(250 mm×4.6 mm, 5 μm) was used for the assay with acetonitrile-0.1% phosphoric acid(25∶75) as the mobile phase. Flow rate was 1.0 ml/min. The column temperature was 30 ℃. The detection wavelength was at 256 nm.Results Quercetin showed good linear relationship within the range of 0.215 0–3.225 μg. The correlation coefficient was 0.999 6.The average recovery was 99.39% with RSD 0.82%(n=6). The repeatability was 1.194 mg/ml with RSD 0.40%. Conclusion The average quercetin content in three batches of Honghuixiang injection was 1.191 mg/ml. This method is simple, rapid and accurate. It can be used for the determination of quercetin in Honghuixiang injection.

关 键 词：高效液相色谱法 红茴香注射液 槲皮苷 

分 类 号：R284.1[医药卫生—中药学]