基于IL-17A/TNF-a信号通路的桔梗汤有效部位群干预COPD模型大鼠的实验研究  

Experimental Study of the Effective Components of Jiegeng Decoction Based on IL-17A/TNF-a Signaling Pathway in Intervention of COPD Model Rats

作　　者：张超云[1] 李宏艺 郝鹏飞[1] 高丽[1] 张仲鸣 Zhang Chaoyun;Li Hongyi;Hao Pengfei;Gao Li;Zhang Zhongming(Nanyang Institute of Technology,Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang 473000,China;Nanyang Zhongjingbaixin Pharmaceutical Co.,Lid.,Nanyang 473000,China)

机构地区：[1]南阳理工学院/河南省张仲景方药与免疫调节重点实验室,南阳473000 [2]南阳仲景百信医药科技有限公司,南阳473000

出　　处：《世界科学技术-中医药现代化》2021年第6期1951-1957,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：河南省科学技术厅科技攻关项目(172102310218):基于MUC5AC、EGFR、TNF-a表达的桔梗汤有效部位群对干预慢性阻塞性肺疾病模型大鼠的实验研究,负责人:张超云。

摘　　要：目的研究桔梗汤不同部位基于IL-17A/TNF-a信号通路对COPD模型大鼠的影响,初步筛选桔梗汤治疗COPD的有效部位。方法建立COPD大鼠模型,将40只SD大鼠分为生理盐水组、模型组、桔梗汤总皂苷组、桔梗汤总多糖组和桔梗汤总黄酮组,经过28天的治疗后,观察分析大鼠一般情况及体重变化、大鼠肺组织病理学改变及气管壁厚度变化、大鼠血清中IL-17A、TNF-a、IFN-α和IFN-β的表达。结果桔梗汤总皂苷和总黄酮对大鼠体重变化、大鼠气管壁厚度变化、大鼠血清中IL-17A、TNF-a、IFN-α和IFN-β的表达和模型组比较有显著性差异(P<0.05),桔梗汤总多糖对上述指标和模型组比较无显著性差异(P>0.05)。结论桔梗汤总皂苷和总黄酮可通过IL-17A/TNF-a信号通路显著改善COPD大鼠肺功能,并初步确定桔梗汤防治COPD的有效部位为总皂苷和总黄酮。Objective To investigate the effect of different components in Jiegeng Decoction on COPD rats based on IL-17 A/TNF-a signaling pathway,and to screen the effective components in Jiegeng Decoction for COPD.Methods Totally 40 healthy rats were selected and randomly divided into 4 groups,including saline treated group,COPD group,total saponins in Jiegeng Decoction,total polysaccharides in Jiegeng Decoction and total flavonoids in Jiegeng Decoction.Body weight change,histochemistry on lung tissue,thickness change of tracheal wall,serum IL-17 A,TNF-α,IFN-αand IFN-βlevel were checked after a 28-day treatment.Results Treatments of total saponins and total flavonoids significantly affected rat body weight,thickness of tracheal wall,serum IL-17 A,TNF-α,IFN-αand IFN-βlevel compared with COPD group(P<0.05).In contrast,total polysaccharides treatment had undetectable effect compared with COPD rats regarding the above measurements(P>0.05).Conclusion Total saponins and total flavonoids in Jiegeng Decoction can significantly improve rat lung functions in terms of IL-17 A/TNF-αsignaling pathways.Therefore,we conclude that the effective components in Jiegeng Decoction for the relief of COPD rats are total saponins and total flavonoids.

关 键 词：桔梗汤 慢性阻塞性肺炎 IL-17A因子 TNF-a因子 有效部位 

分 类 号：R289.5[医药卫生—方剂学]