不同流速对草鱼幼鱼甲状腺激素代谢的影响  

Effects of different water velocities on thyroid hormone metabolism of juvenile Ctenopharyngodon idellus

作　　者：许娜 刘馨璐 汤蓉[1] 张曦 李莉[1] 李大鹏[1] XU Na;LIU Xin-lu;TANG Rong;ZHANG Xi;LI Li;LI Da-peng(College of Fisheries,Huazhong Agricultural University,Hubei Provincial Engineering Laboratory for Pond Aquaculture,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院,池塘健康养殖湖北省工程试验室,长江经济带大宗水生生物产业绿色发展教育部工程研究中心,武汉430070

出　　处：《淡水渔业》2021年第5期106-112,共7页Freshwater Fisheries

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项课题(2019YFD0900303);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-45-24);湖北省水产绿色高质量养殖技术协同推广计划;国家自然科学基金项目(31670521)。

摘　　要：为探讨不同流速对草鱼幼鱼甲状腺激素代谢的影响,本试验以草鱼(Ctenopharyngodon idellus)幼鱼为对象,设置0、0.5、1和1.5 bl/s四个流速对其进行运动训练10周,训练结束后测定了鱼体血清皮质醇、葡萄糖、甲状腺激素(THs)和促甲状腺激素(TSH)含量,及肝脏脱碘酶(ID1、ID2、ID3)含量和甲状腺激素代谢相关基因的表达水平。结果显示:流速提高草鱼幼鱼的生长速度,未显著改变血清皮质醇和葡萄糖的含量,但显著降低甲状腺素(T4)和三碘甲腺原氨酸(T3)含量,显著升高TSH和脱碘酶(ID1、ID2)含量及甲状腺激素代谢相关基因(ttr、tr-α和tr-β)表达水平。结果表明,不同流速的运动训练影响草鱼幼鱼体内THs的合成、转运和代谢各过程,促进T3和T4的转化,进而改变甲状腺激素代谢水平,并促进鱼体生长。A total of 168 juvenile Ctenopharyngodon idellus were treated with four water velocity(0 bl/s,0.5 bl/s,1 bl/s and 1.5 bl/s,respectively)for 10 weeks to explore the effects of different water velocity on thyroid hormone metabolism in fish.The contents of serum cortisol,glucose,thyroid hormone(THs),thyroid stimulating hormone(TSH),liver deiodinase(ID1,ID2,ID3)and the expression levels of genes related to thyroid hormone metabolism were measured.The results showed that water velocity increased the growth rate of juvenile grass carp,which did not change the contents of serum cortisol and glucose,but significantly decreased the contents of thyronine(T4)and triiodothyronine(T3),and significantly increased the contents of TSH.The contents of deiodinase(ID1,ID2)and the expression levels of thyroid hormone metabolism-related genes(ttr、tr-αand tr-β)were significantly increased.The results showed that exercise training at different water velocity affected the synthesis,transport and metabolism of THs in juvenile grass carp,which promoted the transformation of T3 and T4,and then changed the thyroid hormone metabolism and promoted the growth of fish.

关 键 词：草鱼(Ctenopharyngodon idellus) 运动训练 甲状腺激素 脱碘酶 生长 

分 类 号：S917.4[农业科学—水产科学]