西瓜种质抗病性的分子标记检测和人工接种鉴定  

Screening of watermelon germplasm for disease resistance by molecular markers and artificial inoculation

作　　者：易丽聪 王运强[1] 龚钰 邱益纯 焦春海[2] 徐上军 戴照义[1] YI Licong;WANG Yunqiang;GONG Yu;QIU Yichun;JIAO Chunhai;XU Shangjun;DAI Zhaoyi(Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement/Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;Bureau of Agriculture and Rural Affairs of Shishou City,Shishou 434400,Hubei,China)

机构地区：[1]蔬菜种质创新与遗传改良湖北省重点实验室·湖北省农业科学院经济作物研究所,武汉430064 [2]湖北省农业科学院,武汉430064 [3]石首市农业农村局,湖北石首434400

出　　处：《中国瓜菜》2021年第9期7-14,共8页China Cucurbits And Vegetables

基　　金：湖北省重点研发计划项目(2020BBA037);财政部和农业农村部:国家现代农业产业技术体系项目(CARS-25);湖北省农业科学院青年基金项目(2020NKYJJ05)。

摘　　要：病害是西瓜生产中导致产量和品质下降的关键因素。利用已报道的西瓜抗枯萎病、炭疽病和白粉病分子标记对230份西瓜种质资源进行抗病性鉴定,分别筛选出相应的抗性种质60份、20份和35份,其中兼抗枯萎病和炭疽病的资源6份,兼抗枯萎病和白粉病的资源8份,兼抗炭疽病和白粉病的资源6份。对其中23份种质进行抗枯萎病人工接种鉴定,筛选出8份高抗西瓜种质,为西瓜抗病育种提供了宝贵的种质资源。Disease is a crucial reason leading to yield and quality decline in watermelon production.In this paper,three previously reported molecular markers for resistant to fusarium wilt,anthracnose and powdery mildew were employed to detect the potential disease resistance of 230 watermelon germplasm resources,and 60,20 and 35 resistant germplasm were identified,respectively.Also,eight watermelon germplasm that highly resistant to fusarium wilt were obtained by artificial inoculation.This work provides valuable germplasm resources for watermelon disease resistance breeding.

关 键 词：西瓜 种质 抗病性 分子标记 人工接种 

分 类 号：S651[农业科学—果树学]