外源硫化氢对西葫芦幼苗耐盐性及生理特性的影响  

Effects of exogenous hydrogen sulfide on the physiological characteristics under salt tolerance of zucchini seedlings

作　　者：王春林[1] 武芸[2] 刘秀丽[1] 王风琴[1] WANG Chunlin;WU Yun;LIU Xiuli;WANG Fengqin(College of Life Science&Technology,Longdong University,Qingyang 745000,Gansu,China;College of Chemistry and Chemical Engineering,Longdong University,Qingyang 745000,Gansu,China)

机构地区：[1]陇东学院生命科学与技术学院,甘肃庆阳745000 [2]陇东学院化学化工学院,甘肃庆阳745000

出　　处：《中国瓜菜》2021年第9期45-49,共5页China Cucurbits And Vegetables

基　　金：国家自然科学基金(31960375);甘肃省高等学校科研项目(2017A-096)。

摘　　要：为探究外源H2S对西葫芦幼苗耐盐性的调节作用,以西葫芦冬玉99F1为试材,通过基质培养,研究了150 mmol·L^(-1)NaCl胁迫下施加外源H2S供体NaHS(0、0.2、0.4、0.6、0.8、1.0 mmol·L^(-1))对西葫芦幼苗活性氧代谢及渗透调节物质的影响。结果表明,盐胁迫显著增加了西葫芦幼苗H_(2)O_(2)、超氧阴离子、MDA和Pro(脯氨酸)含量,提高了SOD、POD和CAT活性。在盐胁迫下,外施NaHS均可显著提高SOD和CAT活性,仅0.8 mmol·L^(-1)NaHS可显著提高POD活性,且可使3种酶活性分别提高36.74%、41.84%和20.34%;可减少活性氧和MDA积累,0.8 mmol·L^(-1) NaHS可使H_(2)O_(2)、超氧阴离子和MDA含量分别降低9.27%、63.88%和52.66%,Pro含量显著增加。综上所述,外源H2S可通过提高活性氧清除系统的酶活性,减少活性氧和膜脂过氧化产物的生成,并通过调节渗透物质来提高西葫芦幼苗抗盐能力,其中0.8 mmol·L^(-1)NaHS效果最优。To explore the effects of hydrogen sulfide on zucchini seedlings under salt stress,Dongyu 99F1 planted in substrate culture was used as the experimental material,and NaHS with different concentrations(0,0.2,0.4,0.6,0.8,1.0 mmol·L^(-1))were applied.The active of oxygen metabolism and osmotic adjustment substance were investigated.The results showed that H_(2)O_(2),O2-,MDA and proline content of zucchini seedlings were increased,and the activity of SOD,POD and CAT of zucchini seedlings were enhanced under salt stress.Exogenous hydrogen sulfide could increase the SOD,CAT activity and proline content significantly under salt stress,but only 0.8 mmol·L^(-1) NaHS could enhance the POD activity.The SOD,POD and CAT activity were enhanced 36.74%,41.84%and 20.34%respectively after 0.8 mmol·L^(-1) NaHS treatment.and the contents of H_(2)O_(2),O2-,MDA were decreased 9.27%,63.88%and 52.66%respectively compared to salt stress.This study indicated that exogenous hydrogen sulfide could increase salt resistance of zucchini seedlings because it could enhance the activity of antioxidant enzymes and membrane lipid superoxide biosynthesis.The alleviating effect for salt stress under 0.8 mmol·L^(-1) NaHS concentration was best.

关 键 词：西葫芦 硫化氢 盐胁迫 生理特性 

分 类 号：S642.6[农业科学—蔬菜学]