Ni^(2+)和金霉素共存对序批式反应器性能、微生物活性及其微生物群落的影响  

Combined Effects of Divalent Nickel and Chlortetracycline on the Performance,Microbial Enzymatic Activity and Microbial Community in Sequencing Batch Reactors

作　　者：武原原 李姗姗 吴淑妍 潘云浩 于娜玲 赵长坤 高孟春[1,2] Wu Yuanyuan;Li Shanshan;Wu Shuyan;Pan Yunhao;Yu Naling;Zhao Changkun;Gao Mengchun(The Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Qingdao 266100, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2021年第10期89-100,共12页Periodical of Ocean University of China

基　　金：中央高校基本科研业务费专项(201964003)资助。

摘　　要：本文研究了10 mg/L的Ni^(2+)和5 mg/L的金霉素(CTC)对序批式反应器(SBR)性能、微生物酶活性和微生物群落的影响。结果表明,COD去除率在反应器整个运行过程中始终保持在90%以上。10 mg/L Ni^(2+)单独存在及其与5 mg/L CTC共存均能导致氮去除率的下降,且在二者共存时下降程度最大,表明Ni^(2+)和CTC共存对氮的去除呈现协同抑制作用。随着进水中Ni^(2+)和CTC的加入,活性污泥的耗氧速率、硝化和反硝化速率均有所降低。脱氢酶活性以及与脱氮相关的微生物酶活性的变化趋势与耗氧速率和相应的硝化反硝化速率的变化趋势保持一致,均出现了一定程度的降低。Ni^(2+)和CTC共存对硝化反硝化活性呈现协同抑制作用,协同抑制作用主要依赖于Ni^(2+)的含量。Ni^(2+)和CTC共存对活性污泥中活性氧(ROS)的产生和乳酸脱氢酶(LDH)的释放呈现协同促进作用,可以诱导活性污泥细胞内氧化应激以及细胞损伤。长期暴露条件下添加10 mg/L Ni^(2+)和5 mg/L CTC会降低微生物群落的丰富度和多样性。Ni^(2+)和CTC的存在显著抑制了活性污泥中硝化菌(Nitrosomonas、Nitrospira)和反硝化菌(Thauera、Longilinea、Denitratisoma和Anaerolinea)的相对丰度,从而抑制了SBR的脱氮性能。The combined effects of Ni^(2+) at 10 mg/L and CTC at 5 mg/L on the performance,microbial enzymatic activity and microbial community in sequencing batch reactors(SBRs)were investigated.The COD removal efficiency was always above 90%during the whole operational process.The presence of 10 mg/L Ni^(2+) alone and its coexistence with 5 mg/L CTC caused the decrease of the nitrogen removal efficiencies,and their decreased degrees were the highest at the addition of mixed Ni^(2+) and CTC,indicating that the combined effect of Ni^(2+) and CTC had synergistic inhibitory effect on nitrogen removal.The special oxygen uptake rate,special nitrification and denitrification rates of activated sludge decreased with the addition of Ni^(2+) and CTC.The microbial enzymatic activities associated with the nitrogen removal showed similar change trends with the corresponding nitrification and denitrification rates with the addition of Ni^(2+) and CTC.The Ni^(2+) and CTC had synergistic inhibition effects on nitrifying and denitrifying activities.The combined toxicity was mainly dependent on Ni^(2+) level.The presence of Ni^(2+) and CTC promoted the activated sludge to produce more reactive oxygen species and release more lactate dehydrogenase,which could impact on the microbial morphology and physiological functions.The functional groups of sludge stressed by Ni^(2+) was altered more obvious than that by CTC.The richness and diversity of microbial community decreased with the addition of 10 mg/L Ni^(2+) and 5 mg/L CTC.The presence of Ni^(2+) and CTC significantly inhibited the relative abundance of nitrobacter(Nitrosomonas,Nitrospira)and denitrifiers(Thauera,Longilinea,Denitratisoma and Anaerolinea)in activated sludge and further inhibited the denitrification performance of SBR.

关 键 词：Ni^(2+) 金霉素 微生物酶活性 微生物群落 序批式反应器 

分 类 号：X703[环境科学与工程—环境工程]